Team:Tufts/Description
Description
Our project's goal is to build a current-based biosensor using transformed, metal-reducing Shewanella oneidensis. Ultimately, specific compounds will be tested in their ability to activate the current-producing pathway of Shewanella, to test the transformed bacteria's ability to work as a quantitative analyst with measurable current intensity.
The Process
- First, a Gibson assembly will be performed to construct an inducible mtrB plasmid
- PCR and electrophoresis will isolate and confirm the DNA
- Electroporation will be used to introduce the sequence into E. coli
- Conjugation will be used to introduce the sequence into Shewanella
- Current responses of S. oneidensis to various inducers will be measured
Inspirations
- We found Cornell University's impressive project on Shewanella current production after discussing possible project ideas for a semester in our brainstorming forum. It went along with our idea to create a biosensor, building off the goals of our Tufts 2018 iGEM project (an miRNA toehold trigger).
- 2012 Cornell University