Characterization
Our major contribution this year was to add an OriT site to the plasmid from Cornell 2012
- This addition allowed us to conjugate into Shewanella, using the RK2 carrying strain of E. Coli
Our next steps are to test this plasmid with a range of analytes, including Mercury and Arabinose
Mercury
![](https://static.igem.org/mediawiki/2019/4/45/T--Tufts--MercPlasmid.png)
Arabinose
![](https://static.igem.org/mediawiki/2019/6/63/T--Tufts--AraPlasmid.png)
These constructs were designed to create electrical potentials in the presence of Mercury or Arabinose, respectively