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Medal Requirements

Bronze Medal Requirements

Register and attend:
We Registered for iGEM, had a stunning summer full of new experiences. For some proof, visit our gallery!We are currently very excited and looking forward to participating in the giant jamboree.
Deliverables:
We have delivered all of the required deliverables: Wiki, Poster, Presentation and Judging Form. We can’t wait to present our project and poster in Boston.
Attributions:
We have created a wiki page to acknowledge and thank all of those who have helped and supported us throughout this project. Without their help, this project would have never been possible. For more information check out our attributions page.
Project Inspiration and Description:
We have created a wiki page describing our project, our inspiration and the goals we want to achieve. For more information check out our project description page.
Characterization:
We have characterized the secretory signal peptide AmyE (BBa_K2273023), with which we showed the secretion and extracellular activity of our new part, the Glucose Oxidase (GOx) enzyme (BBa_K2934000). Check out our design and lab results for more information.

Validated Parts:
We have added a total of nine new parts to the part registry, six of them are new basic parts (basic parts page) and three are composite parts (composite parts page). Among these parts, we have experimentally showed that 3 parts (BBa_K2394000, BBa_K2394001, BBa_K2394004) work and therefore are considered validated.
Collaboration:
We collaborated with 11 iGEM teams from 8 different countries. Check out our Collaboration page for more information!
Human Practices:
We considered the practical implications of our project by consulting with experts from our community as well as interacting with the public. Furthermore, we believe in educating the future generation, and therefore, we have arranged a Hackathon event revolving around the subject of sustainability. We also wanted to bring our iGEM community closer and invented the "One Minute Challenge". Have a look at our Human Practices page.

Integrated Human practice:
We have consulted many experts and interacted with the public, all of which lead to important and meaningful changes within our project. We have also used and promoted commercial nectar solution and SBP capsules, presenting our project to the honey and biotech industries and receiving useful information which leads to the refining of our lab experiments. For more information visit our Integrated Human Practice page.
Model Our Project:
We have modeled our system, which mimics the honey-making process performed in the honey stomach of the bee, gaining new information which helped us gain a better understanding about our system and adjust our wet-lab experiments accordingly. We have considered the transcription of the Honey Circuit, the enzymes kinetics and secretion and the influences of pH change on enzyme activity and bacterial growth. Additionally, we helped the BGU team to model how their pesticide-bacteria would affect the entire population of the mosquitos in the afflicted area.
Demonstrate Our Work:
Herewe show that our system works as expected. To do so, we have performed a series of experiments testing the functionality of each component of our system individually, forming the "BeeFree" system prototype.

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