Team:Tacoma RAINmakers/Parts
Team:ECUST/Lab/Notebook
Parts Overview
Part Table
<groupparts>iGEM19 Tacoma_RAINmakers</groupparts>OtsA encodes trehalose-6-phosphate synthase (TPS), which is part of the first step in the most common biosynthetic pathway to create the disaccharide, trehalose. The second step is catalyzed by trehalose-6-phosphate phosphatase (TPP). TPS “catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate” (UNIPROT). Trehalose is made in many different biological systems, like bacteria and fungi, but is less common in higher eukaryotes. It is important because of its role in protecting organisms against abiotic stressors, like drought, cold, heat, desiccation, and salt (Iordachescu and Imai, 2011). Although the mechanism is not fully understood, Rhizobia that overexpress OtsA has been shown to improve the growth and stress tolerance of the legumes in which they colonize (Suarez et al. 2008). OtsA therefore has the potential to be used in improving agriculture practices.
Source Sequence:
GenBank AY731700.1 with 6 silent mutations made to decrease GC content during DNA synthesis. 4 illegal PstI sites and 1 illegal EcoRI site removed.
References:
- Iordachescu M. and Imai R. (2011). Trehalose and Abiotic Stress in Biological Systems, Abiotic Stress in Plants - Mechanisms and Adaptations, Prof. Arun Shanker (Ed.), ISBN: 978-953-307-394-1, InTech, Available from: http://www.intechopen.com/books/abiotic-stress-in-plants-mechanisms- andadaptations/trehalose-and-abiotic-stress-in-biological-systems
- Suarez R. Wong A. Ramirez M. Barraza A. Orozco M. D. C. Cevallos M. S. Lara M. Hernandez G. Iturriaga G. (2008) Improvement of drought tolerance and grain yield in common bean by overexpressing trehalose-6-phosphate synthase in rhizobia. Molecular Plant-Microbe Interactions, 21 7 (July 2008), 958 966 , 0894-0282
- UNIPROT https://www.uniprot.org/uniprot/P31677. Accessed Sept 20, 2019.