Team:TU Darmstadt/Project/Primer and Backbone List
Backbone List
During our work in the wetlab we used a variety of different plasmid backbones for cloning and expression of our BioBricks. You can find a list of all the plasmids we used and the corresponding parts below.
| Name | Resistance | Size | Features | Used for the following parts |
|---|---|---|---|---|
| pACYCT2 | Cmp | 3975 bp | cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, T7 promoter, lac operator, T7 terminator | BBa_K3187000, BBa_K3187001, BBa_K3187003, BBa_K3187004 |
| pDESTara2 | Amp | 4556 bp | AmpR, araBAD promoter, araC, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, rrnB T1 and T7Te terminator promoter, lac operator, T7 terminator | BBa_K3187010 |
| pET24 | Kan | 5236 bp | KanR, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, f1 ori, lacI repressor + lacI promoter, T7 promoter + lac operator, 6xHis, T7 terminator | BBa_K3187000, BBa_K3187002, BBa_K3187005, BBa_K3187006, BBa_K3187008 |
| pSB1C3 | Cmp | 2070 bp | CmR, lambda t0 terminator, high-copy-number ColE1/ pMB1/ pBR322/ pUC ori, his operton Terminator, BioBrick Pre- and Suffix | BBa_K3187014, BBa_K3187015 |
| pTeTW3con2 | Cmp | 4555 bp | cat promoter, CmR, p15A ori, lacI repressor + lacI promoter, tetR/tetA promoter, tetR repressor, tet operator, T7 promoter + lac operator, T7 terminator | BBa_K3187011, BBa_K3187012, BBa_K3187013 |
