We've used several primers to amplify and alter our bricks that we have. Below you can find a whole list of primers sorted by their use!
Amplification primers
| Name | Sequence |
|---|
| Amplification1_F | GACGCCAGGTTGAAT-GAATTCGCGGCCGCTT |
| Amplification1_R | CCGTATTTGGAGGTA-CTGCAGCGGCCGCTA |
| Amplification_Brick2_F | GACGCCAGGTTGAAT-GAATTCGCGGCCGCTTCTAGAACATTGATTATTTGC |
| Amplification_Brick2_R | CCGTATTTGGAGGTA-CTGCAGCGGCCGCTACTAGTAGCT |
Sequencing primers
| Name | Sequence |
|---|
| Seq_P1_R | GAGTTCGAAATCGAAGGTGAAGG |
| Seq_P1_F | CCTTCACCTTCGATTTCGAAC |
| Seq_P2_R | TTTATCGCCGCCAACAATTTG |
| Seq_P2_F | TTAGATGGTGATGTTAATGGGC |
| Seq_P3_F | TTAGATGGTGATGTTAATGGGC |
| Seq_P3_R | GTTGCATCACCTTCACCCTCTC |
| VF2 | TGCCACCTGACGTCTAAGAA |
| VR | ATTACCGCCTTTGAGTGAGC |
New amplification primers
| Name | Sequence |
|---|
| Max_primer_BB1/BB1.2_F | GACGCCAGGTTGAAT-GAATTCGCGGCCGCTTCTAGAATTTCAG |
| Max_primer_BB1/BB1.2_R | CCGTATTTGGAGGTA-CTGCAGCGGCCGCTACTAGTATGG |
| Tobi_BB2_F | GACGCCAGGTTGAAT-GAATTCGCGGCCGCTTCTAGAACATTGATTATTTGC |
| Tobi_BB2_R | CCGTATTTGGAGGTA-CTGCAGCGGCCGCTACTAGTAGC |
| Tobi_BB3_F | GACGCCAGGTTGAAT-GAATTCGCGGCCGCTTCTAGAGACTATCTTC |
| Tobi_BB3_R | CCGTATTTGGAGGTA-CTGCAGCGGCCGCTACTAGTAAGG |
Overlap extension and HIFI primer
| Name | Sequence |
|---|
| OE_1_1+2_F_0008 | CGCCAGGTTGAATGAATTCGC |
| OE_1_1+2_R_0009 | CAAATAATCAATGTTGGCCGGCTTGACGG |
| OE_2_1+2_F_00010 | GTCAAGCCGGCCAACATTGATTATTTGCACGGCGTCAC |
| OE_2_1+2_R_00011 | GTATTTGGAGGTACTGCAGCGG |
| OE_1_1+3_F_00012 | CGCCAGGTTGAATGAATTCGC |
| OE_1_1+3_R_00013 | CGAGAAGATAGTCTTGACGGCTAGCTCAGTCCT |
| OE_3_1+3_F_00014 | AGCTAGCCGTCAAGACTATCTTCTCGCTTATCGTGTT |
| OE_3_1+3_R_00015 | GTATTTGGAGGTACTGCAGCGG |
| OE_1.2_1.2+3_F | CGCCAGGTTGAATGAATTCGC |
| OE_1.2_1.2+3_R | GCGAGAAGATAGTCGGCACATTGATTATTTGCACGG |
| OE_3_1.2+3_F | ATAATCAATGTGCCGACTATCTTCTCGCTTATCGTGTTTGACA |
| OE_3_1.2+3_R | GTATTTGGAGGTACTGCAGCGG |
New OE primers
| Name | Sequence |
|---|
| OE_2_1+2_F_00010 | GTCAAGCCGGCCAACATTGATTATTTGCACGGCGTCAC |
| OE_2_1+2_R_00011 | GTATTTGGAGGTACTGCAGCGGC |
HIFI primers AraC3 Linearization Vectors
Linearization for Vector to be complementary to OE primer amplified BB (OE)
| |
|---|
| Vector_openingRBB1_1.2_2_3 | CGAATTCATTCAACCTGGCGTC-CGGGTATGGAGAAACAGTAGAGAGTTGC |
| Vector_openingFBB1_1.2_2_3 | GCTGCAGTACCTCCAAATACGG-CGCGTTATGCAGGCTTCCTCGC |
| |
|---|
| |
| Vector opening 1.2+3 F | CTAGGACTGAGCTAGCCGTCAAGCC |
| Vector opening 1.2+3 R | GTTGAACAGTACGAACGTGCCGAGG |
| Delete BB2 F | TTTGTAACAGCTGCTGGGATTACACATGGC |
| Delete BB2 R | CGGCTTGACGGCTAGCTCAGTCC |
Linearization for Vector to be complementary to Amplified BB (BB)
| |
|---|
| Vector_openingRBB1_1.2_2_3 | CGAATTCATTCAACCTGGCGTC-CGGGTATGGAGAAACAGTAGAGAGTTGC |
| Vector_openingFBB1_1.2_2_3 | GCTGCAGTACCTCCAAATACGG-CGCGTTATGCAGGCTTCCTCGC |
Deletion primer
| Name | Sequence |
|---|
| BB1 F | CTAGGACTGAGCTAGCCGTCAAGCC |
| BB1 R | GTTGAACAGTACGAACGTGCCGAGG |
| BB2 F | TTTGTAACAGCTGCTGGGATTACACATGGC |
| BB2 R | CGGCTTGACGGCTAGCTCAGTCC |
Primer to add RBS
| Name | Sequence |
|---|
| RBS3 to RFP F | AGCCATCTAGTACGTCATGTCTTTCTTATTATTTGTCGTCGTCG |
| RBS3 to RFP R | ATGTGACCGACTGTGAATCTG |
| RBS3 to GFP F | ACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCC |
| RBS3 to GFP R | ACGTCATGTCTTTCTTATTACAGATCTTCTTCGCTAATCAGTTTCTG |
NO-Sensing System Primers
| Name | Sequence |
|---|
| Amp1 | ATCGCTAATGTGAGCCCATC |
| Amp2 | ATGTGACCGACTGTGAATCTG |
| Cutout F | CCCATCGTGGTCCTCAGATA |
| Cutout R | CTGCAGCGGCCGCTACTAGTAGTACTTCGCCACTAATTCCACA |
| Sens_Opti F | ATCGCTAATGTGAGCCCATCGTGGTCC |
| Sens_Opti R | CAATGTGACCGACTGTGAATCTGCAGCGG |
| Alternative_1 | CAGATATGAATTCGCGGCCGCTTCTAGAGTGTGGAATTAGTGGCGAAGTACGATTCC |
