Team:Shanghai YGQ/Notebook

Shanghai_YGQ

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Notebook

Plasmid Design and Validation

Preparation of Competence

Verification of Competence Success

Design Primer

PCR Amplification Adding Enzyme-cut Site

Construct Plasmid

Import

Design the gene circuit with the original quorum sensing system of Acetobacter xylinum, and collect data on ginA (Acetobacter xylinum needs electrotransfer)