Team:PuiChing Macau/Demonstrate
Demonstration
From our results, we can prove the feasibility of our system. The two laccases we have modified have been shown to degrade both EDCs and dyes, and the signal peptides they carry also function properly, continuously releasing laccase into the water, especially K3021004 proved to be abundant in the supernatant.
Results
Our purposed system utilizes the engineered E.coli which is proved to be effectively and stably producing laccases that are able to degrade some type of EDCs. The system is viable in a realistic situation as in the modelling section stated, the chosen laccase K3021004 (NSP4_Lac1326_HisTag) performs well in a wastewater environment. In a simulated wastewater environment (pH 6, Temp 25°C), the laccase is predicted to maintain around 80% of its maximal activity. In high salinity environments, data has shown the enzymes would even have enhanced performances.
In the Hardware section, our team proposed a rotating biological contractor model for our modified bacteria to interact with waster water. Many advantages have been stated including the compact size advantage, easy construction and the fact that it is not susceptible to insects and the humid and hot summer in Macau.
Hardware
Overall, our investigation has shown us the potentials and possibilities in developing such a system that would perhaps provide solutions to EDC contamination in industrial and household waster water.