Team Attributions

In terms of our project, we have utilized engineered E.coli BL21 (DE3) to produce our selected Laccases.(Lac1326 and K863030) by cloning a collection of Laccases into E. coli, which include a stress-tolerant laccase. To enhance the EDC degradation efficiency, we also added a secretion signal peptide, NSP4 to the laccases expressed in E. coli. Moreover, we further optimized our design by transforming the laccases(LAC1326), with a pilA secretion signal peptide, into cyanobacteria (Synechococcus sp) in order to improve the sustainability of the EDC degradation system, In addition, we also designed a water filter that fits our engineered bacteria.

As for characterization, we have chosen BBa_K863000 and conducted a decolorization experiment. The results showed that K863000 was the most efficient enzyme with 81.09% removal of indigo carmine (initial concentration of 10 mg/L) after 96 hours incubation in ABTS at 37°C.

Students

Chon Hou
(Benny)
LAO

Student leader, outreach

Jeremy HU

Lab work, outreach, wiki page, and result analysis

Franklin
YEUNG

Media leader, developer of wiki page, outreach

Sin U
(Alice)
KONG

Outreach

Kin Tong
(Rex)
PAO

Fundraising, outreach, and treasurery

Yating
(Sandy)
MO

Lab work leader, outreach, result analysis and lab log

Ieng Chon
(Chloe)
LI

Lab work, safety form and outreach

Chon Neng
(James)
LEONG

Outreach leader

Chong Chon
(Jones)
CHENG

Outreach

Hou Ip
(Donald)
LAO

Labwork, outreach

Weng I
(Alisa)
LEONG

Outreach

Yi Fan
(Linda)
XIANG

Lab work, designer, outreach

Sau Wa
(Donna)
AU

Lab work, lab log and outreach

Hoi Man
(Karina)
YUEN

Lab work, outreach, brochure design and results analysis

Lok Hang
(Lok)
CHIU

Banner, T-shirt and team logo design, modelling, lab work and outreach

Chi Hou
(Herman)
LEONG

Filter model design, lab work and outreach

Primary investigators

Wai Man CHEONG

Facilitated the team activities, and ensures the plan can be implemented as far as practicable. The works include coordinating with advisors and labs for experiments, organizes outreach and fundraising activities, etc.