Team:Northern BC/Parts
Parts
Our intention was to characterize aeblue chromoprotein expression in a yeast system given that previous characterization has been carried out in bacteria. In order to do this, we had to modify a yeast shuttle vector prior to ligating the aeblue gene into the vector. This included making two site directed mutations to eliminate BioBrick prefix and suffix restriction sites from elsewhere in the vector. Additionally, we added the BioBrick prefix and suffix to the yeast shuttle vector for RFC-10 compatibility (see our Results page). Unfortunately, we have not yet successfully ligated aeblue into the vector for subsequent expression and characterization.
