General Lab Rules and Guidelines:
All team members had to receive training included the laboratory machinery and the use of chemicals, especially toxic drugs before started our project in the lab. Moreover, we thought carefully about and managing any risks to ourselves, our colleagues, community and the environment.
About the Instruments:
1. Always close and latch completely the freezer doors (both–80℃ and –20℃) when not in use, and make sure the fridge is closed as well.
2. Note on the records of instruments the person in charge should check as followed:
(1) carefully fills in the operating rules and use records.
(2) train the user seriously and use it in strict accordance with the operating rules of the instrument.
(3) responsible for the opening and sharing of large precision instruments.
(4) responsible for the daily and maintenance of large precision instruments.
(5) responsible for the safety of the use of large precision instruments.
(6) conscientiously protects the manual for the use of large instruments and other random data.
About Ourselves:
1.If you notice that a general lab supply is running low, write the item description, quantity desired along with your name and the date. Try to write items up far enough in advance to ensure that we can restock our supplies before running out.
2. Please notify the lab manager of any problems noticed as soon as possible.
3. Never wear gloves outside of the lab (please remove your gloves before going into the computer room or the offices).
4. Please adjust the pipette to full range back to the rack and return all chemicals to the shelves.
5. Make sure samples are capped tightly and counterbalanced appropriately when centrifuging. Also, be sure the screw the top on the microcentrifuge during use.
6. If you borrow something from someone else's bench, please return it immediately after you finish using it.
7. If you break something, please report it to the person whose lab duty it is.
8. You are not allowed to eat, drink or fight in the lab.
9. If injured, use medical first aid kit in time. In serious condition, seek medical advice with others.
About the Hazardous Chemical Accidents:
1. After the occurrence of hazardous chemical accidents, the personnel on the spot first carry out personal protection, and then take the corresponding on-site disposal measures according to different types of accidents, and then take the corresponding internal and external contact after judging the grade and trend of the accident.
2. fire disposal procedures for laboratory chemicals:
If there is a fire, we must not be panic and should keep calm. First of all, all indoor fire sources and power should be cut off immediately. Then according to the specific situation, the rescue and fire extinguisher should be carried out correctly. The common methods are as follows:
(1) flammable liquid fire: immediately remove the cut combustible material in the ignition area, shut down the ventilation facilities, and prevent the expansion of combustion. If the fire area is small, you can cover it with rags, wet cloth, iron sheets or sand to isolate the air and extinguish it. When covering, the action should be light, avoid smashing or knocking over the glass utensils filled with flammable solvents, resulting in more solvent outflow and expanding the fire surface.
(2) Fire of alcohol and his soluble liquid in water: water may be used to extinguish fire.
(3) organic solvents ignition: use cotton cloth or sand to extinguish. Water must not be used, otherwise it will expand the combustion area.
(4) when clothes catch fire, do not run around the body with clothes, coats, etc., or lie on the ground rolling to put out the fire.
About the Environment:
1. We used E. coli as a chassis creature with no harm to human body and environment.
2. No viruses will be used.
3. Our strains involved in this experiment do not produce any toxic substances.
4. Waste bacteria solution and culture medium will be sterilized before being discarded.