Team:NEFU China/Basic Part
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Basic Parts
| Name | Number | Type | Description | Length |
|---|---|---|---|---|
| P-T7 | BBa_K2913015 | Promoter | A strong promoter. | 19 bp |
| ECF-20 | BBa_K2913016 | Coding | An active transcription factor involved in initiation. | 582 bp |
| HucR | BBa_K2752003 | Coding | A repressor that is inhibited by uric acid. | 915 bp |
| Pluc | BBa_K2913003 | Promoter | This promoter consists of the 18-bp HucR binding sequence located between −35 and −10 motifs of Lac promoter. It is a part of the composite part Phuc1 (BBa_K2913005) and Phuc3 (BBa_K2913007). | 30 bp |
| Uricase | BBa_K2158000 | Coding | Uricase from Bacillus subtilis. | 1485 bp |
| LacI | BBa_C0012 | Coding | LacI repressor from E.coli (+LVA). Coding region for the lacI protein with an LVA degradation tail and without an RBS. LacI binds to the Plac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to lacI and inhibits its operation, therefore promoting transcription. A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part. | 1153 bp |
| PfnrF8 | BBa_K2913009 | Promoter | This is a hypoxia-inducible promoter to restrict gene expression specifically in the hypoxic environment of tumors. The transcriptional activation is promoted by the PfnrF8 needs a specific transcription factor called FNR, which can bind to oxygen. When the oxygen level is at a normal range in the internal environment, the transcription factor FNR will associate with oxygen, leading to its impeded binding to the PfnrF8 promoter, and reduce the transcription of its downstream gene. However, in the hypoxic environment of tumors, FNR will disassociate with oxygen and activate its downstream gene. | 51 bp |
| Anti-ECF20 | BBa_K2913018 | Coding | A protein that inhibits the initiation of transcription of ECF-20 | 429 bp |
| TNF-α | BBa_K2913019 | Coding | Tumor necrosis factor (TNF, cachexin, or cachectin; once named as tumor necrosis factor alpha or TNFα) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.TNF is a member of the TNF superfamily, consisting of various transmembrane proteins with a homologous TNF domain. The primary role of TNF is in the regulation of immune cells. TNF, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1- & IL6-producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, psoriasis and inflammatory bowel disease (IBD). Though controversial, studies of depression and IBD are currently being linked to increased levels of TNF. Recombinant TNF is used as an immunostimulant under the INN tasonermin. TNF can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated. | 1383 bp |
| P-Anderson | BBa_J23117 | Promoter | The promoter parts can be used to tune the expression level of constitutively expressed parts. | 35 bp |
| lldPR | BBa_K1847016 | Coding | Regulatory protein lldR together with L-lactate permease (lldP). | 2443 bp |
| P-BAD | BBa_K2913022 | Promoter | The P-BAD expression system allows tightly controlled, titratable expression of your protein through the regulation of specific carbon sources such as glucose, glycerol, and arabinose. P-BAD is ideal for expressing toxic proteins and optimizing protein solubility in E.coli. | 330 bp |
| ftnA-M | BBa_K2913024 | Coding | In order to enhance the tumor-targeting ability of our engineered bacteria, we select a mutant ferritin, ftnA-M including H34L + T64I, to create a magnet responding cassette, so that we can reduce the whole bacteria mass in our project, minimizing the potential harmful effects of bacteria on the human body. It is doubly mutated from wild type ferroin ftnA protein at the “B-channel” that transports iron, likely enlarging the B-type channel, exhibited the strongest ferromagnetism. | 498 bp |
| Pecf20 | BBa_K1461001 | Promoter | This part is from Pseudomonas Fluorescens sigma 70 family. ECF20 (BBa_K1461004) activates the expression of the downstream genes of this promoter. | 80 bp |
| Cytosine deaminase | BBa_K2913021 | Coding | An enzyme that oxidizes 5-flucytosine to 5-fluorouracil. | 1284 bp |
| YgfU | BBa_K2334004 | Coding | YgfU is a high-capacity transporter for uric acid in E.coli, which is homologous to nucleobase transporters of the ubiquitous family NCS2. | 1715 bp |
| TrrnB | BBa_K2913020 | Terminator | A terminator works well. | 158 bp |
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iGEM-NEFU_China2019
E-mail:nefu_igem2019@163.com
No.26 Hexing Road, Xiangfang District, Harbin, Heilongjiang Province 150040
