Team:NEFU China/Composite Part
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Composite Parts
| Name | Number | Type | Description | Length |
|---|---|---|---|---|
| Phuc1 | BBa_K2913005 | Promoter | One of the series of Phuc. This composite part consists of Pluc (BBa_K2913003), conjugation sequence (BBa_K2913002), and a random sequence as long as HucO sequence. Phuc1 includes one HucR binding site inside the Pluc (BBa_K2913003). In the absence of uric acid (or xanthine), HucR protein binds to Pluc and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-Pluc binding, RNA polymerase initiates a transcriptional process of downstream genes. | 92 bp |
| Phuc2 | BBa_K2913006 | Promoter | One of the series of Phuc. This composite part consists of Plac (BBa_K2913004), conjugation sequence (BBa_K2913002), and HucO sequence (BBa_K2913001), which is the only one HucR binding site of Phuc2. In the absence of uric acid (or xanthine), HucR protein binds to HucO and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-HucO binding, RNA polymerase initiates a transcriptional process of downstream genes. | 81 bp |
| Phuc3 | BBa_K2913007 | Promoter | One of the series of Phuc. This composite part consists of Pluc (BBa_K2913003), conjugation sequence (BBa_K2913002), and HucO sequence (BBa_K2913001). Phuc3 includes two HucR binding site both in the Pluc and HucO. In the absence of uric acid (or xanthine), HucR protein binds to Pluc as well as HucO and prevents the binding of RNA polymerase. When uric acid (or xanthine) antagonizes the HucR-Pluc & HucR binding, RNA polymerase initiates a transcriptional process of downstream genes. | 81 bp |
| lldRO1-PfnrF8-lldRO2(Phll) | BBa_K2913023 | Promoter | Response to high lactate and hypoxia. This is a composite part consists of lldRO1-J23117-lldRO2 (BBa_K1847008) and PfnrF8 (BBa_K2913009). It has a functional improvement of lldRO1-J23117-lldRO2 via tansforming a specific Anderson promoter (BBa_J23117) to a hypoxia-inducible promoter, PfnrF8. The lldRO1--lldRO2 was utilized with other parts of the lldPRD operon (previously named as lct), responsible for aerobic L-lactate metabolism. The lldPRD operon consists of three genes that form a single transcriptional unit inducible by growth in L-lactate. The three genes lldD, lldP and lldR encode a dehydrogenase, a permease and a regulatory protein, respectively. We chose lldR and lldP to assist building our lactic acid response unit. lldR protein can bind to the operators O1 and O2 located on each side of the PfnrF8. When lactic acid level is low, two lldR molecules will individually bind to the O1 and O2 sites and form a tetramer to make DNA strand form a hairpin structure, which can turn off the expression of the downstream gene. When lactic acid level reaches a certain point, lldR will be released from the O1 and O2 operators, and the DNA hairpin will be resolved, leading to activated transcription of the downstream gene. We also used lldP to increase the sensitivity of our system to lactic acid alteration. | 159 bp |
| lldRO1-J23117-lldRO2 | BBa_K1847008 | Promoter | Promoter regulated by lldR. | 143 bp |
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