In this project we respect all of the safety guidelines required by the iGEM foundation. Furthermore, we participated in the RIVM Safe-by-Design grant, which brought safety issues in the design of our project to our attention. We even created an infographic in which we demonstrated different safety aspects of our project. Our project will be using existing technology in a novel way to improve our systems detection mechanism.
Manual:
Step 1.
Make YPD plates
Step 2.
Streak-plate the cells on selective YPD plates
Step 3.
Take several colonies for inoculation in YPD media
Step 4.
Induce mutation mechanisms by centrifuging cells down and adding a 2% galactose rich media with target molecule of interest
Step 5.
To detect functionalized receptors use a flow cytometry set-up for single cell quantisation
Step 6.
Replate bacteria that were positively selected on a new YPD selective growth plate.
Step 7.
Inoculate several colonies for Overnight culture
Step 8.
Use Miniprep Kit to extract DNA for sequencing
Step 9.
Upload sequence to the ROCCloud
