Team:IISER-Pune-India/Composite Parts

Mutatis Mutandis

Parts Overview

Composite Parts

Biobrick Number Part Name Description Designed by
BBa_K3285000 CP+RBS+YF1+RBS+FixJ+TT+pFixK2+RBS+RFP This is a Blue light repressible promoter in which the expression of gene downstream of it will be expressed as a function of intensity of blue light. With increase in intensity of blue light, rfp outpur reduces. Pranav
BBa_K3285334 AraC-pBAD + RBS+ mutD5 + TT This is an Inducible system for tunaebility of mutation rates where increasing concentrations of mutD5, which is a faulty subunit of DNA polymerase III, the mutation rates in bacteria increase due to faulty 3'-5' proofreading. Utkarsh
BBa_K32850020 CI lam + RBS+ RFP+ CIts2+TT This system causes temperature sensitive expression of RFP. At different temperatures, the binding affinity of CIts2 vs CI repressor is lower in opens oppertunities for regulated negetive feedback loops for controlled gene expression. Sayantan
BBa_K3285666 CI lam + RBS+ RFP+ CI +TT This is the promoter-repressor system of pLambda and CI. CI repressor repressor CI lam to make a negetive feedback loop that causes a limited expression of the gene downstream of CI lam and doesn't allow the protein production over a specific limit. Sayantan
BBa_K3285669 Biosensor BBa_K1758333 with new RBS This is the modified Lead biosensor with a different RBS (BBa_B0032) downstream of the pbrAP promoter to improve the sensitivity of the existing biosensor BBa_K1758333 to varying lead concebtrations. Pranav