Team:Hong Kong JSS/Composite Part


Hong Kong JSS

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Composite part










In our project, we had designed one composite part for the expression of Corynebacterium glutamicum metallothionein gene (CgMT) (BBa K3076803) in BL21(DE) E. coli. Metallothionein is a metal chelating protein which suits our aim of creating an E. coli that has increased ability to absorb metal pollutants in water.

Metallothionein is a small, cysteines rich protein. The thiol group of the cysteines can bind heavy metal ions.


The expression of the coding sequence of CgMT(BBa K3076100) was regulated by the
Strong T7 promoter (BBa I719005),
Lac Operator (BBa K3076802),
Strong RBS (BBa K3076801).
The T7 terminator (BBa K395601) was used to terminate the expression.

The design of the composite part for the expression of CgMT (BBa K3076803).