This year, iGEM Bulgaria has submitted the following parts:
BBa_K3208000 a composite part that is an expression cassette for heterologous production of AMPs in E. coli. It contains an N-terminal DAMP4 tag for efficient expression and simple purification of the AMPs. The cloning of new peptide sequences can be done using the two Eco31I sites that allow scarless insertion.
BBa_K3208001 to BBa_K3208009 – expression modules for putative antimicrobial peptides isolated from the genome of the great white shark (Carcharodon carcharias).
BBa_ K3208010 a red expression cassette that can be inserted between two Eco31I sites. This modification can significantly decrease the number of colonies that have to be screened to find a correct transformant when using vectors that rely on Eco31I digestion and ligation.
BBa_ K3208011 a modified version of part BBa_K3208000 with part BBa_ K3208010.
BBa_ K3208012 improved version of part BBa_K2515002 from 2017. The old gRNA cloning vector is modified in a way to contain a red expression cassette between the two Eco31I sites. This modification significantly decreases the number of colonies that have to be screened to find a correct transformant.
