Team:Bulgaria
iGEM Bulgaria
presents
Peptidator P-800
Antibiotic resistance is one of the most urgent threats to public health.
If no action is taken, multiresistant bacteria could cause 10 million deaths each year by 2050 according to the UN Interagency Coordination Group (IACG) on Antimicrobial Resistance.
One of the most promising candidates for battling pathogens is natural organic compounds, like antimicrobial peptides.
Our novel synthetic platform for high throughput isolation and characterization of peptides with antimicrobial properties serves as The Terminator for multi-resistant bacterial pathogens.
We used the available genomic sequencing data as a source of novel peptide sequences that can be used instead of antibiotics. To identify such elements, we used different versions of the BLAST algorithm and known antimicrobial peptides as quarries. Our sequences are isolated from Carcharodon carcharias or great white shark.
The next step would be to have these exact sequences synthesized as an oligonucleotide pool and cloned like an expression library in E.coli.
Last but not least, the activity of this library is tested against a selected group of indicator strains that represent most of the major important human and animal pathogens as to find the perfect Peptidator.