Team:Bulgaria/Labbook
Project Lab book
September
Week 1 (02.09 - 08.09) - first week of wet lab work
Safety instructions; bacterial cultivation; plasmid mini prep; test PCR; test cloning with pSB1C3
Week 2 (09.09 - 15.09)
Design of arabinose induced module that controls the expression of a test substrate - chromoprotein cassette. Quantifying the cloning success ratio when using the gRNA expression vector (see Contribution and Improvement).
Week 3 (16.09 - 22.09)
Cloning of the DAMP4 expression module into the arabinose controlled expression vector. Colony PCR identification of correct transformants. Quantifying the cloning success ratio when using the gRNA expression vector (see Contribution and Improvement).
Week 4 (23.09 - 29.09)
Induction and expression experiments. Test SDS-PAGE to evaluate the functionality of our vector. Designing the primers for cloning the red chromoprotein expression cassette.
October
Week 1 (01.10 - 06.10)
Design of expression modules for putative AMPs from the great white shark. Cloning of the new red chromoprotein expression cassette into the 2 vectors.
Week 2 (07.10 - 13.10)
Quantifying the cloning success ratio when using the gRNA expression vector with red chromoprotein expression cassette.
Week 3 (14.10 - 20.10)
Cloning of the AMP expression modules into pSB1C3. Induction of the expression of the putative AMPs and monitoring the cell survival ability via spectrophotometric measurements.