Team:OUC-China/Parts2
This year, OUC-China constructed many well-characterized parts based on our standardized design principle. We hope our parts will make it easier and more efficient for synthetic biologists and future iGEM teams to get the predictable results during using riboswitch. Our parts include six different Tuners so that they can be easily incorporated into a user circuit of interest when constructing modular riboswitch. We also include many modular riboswitches by utilizing four kinds of riboswitches. Additionally, many composite parts was constructed to verify our design principle of modular riboswitch.(named “RiboLegoes” )
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| Mingyu Yang | Peng Deng | Haofeng Chen | ||
| K2904002 | Regulatory | Tuner C | ||
| K2904003 | Regulatory | Tuner D | ||
| K2904004 | Regulatory | Tuner E | ||
| K2904005 | Regulatory | Tuner S |
asRNA
To control the riboswitches without medium replacement for removing the inducer, we used the model to design different asRNAs which target different regions to activate or deactivate the riboswitch. These parts can achieve these functionality that expand another method to regulate on-off state of riboswitch besides the ligand.
To control the riboswitches without medium replacement for removing the inducer, we used the model to design different asRNAs which target different regions to activate or deactivate the riboswitch. These parts can achieve these functionality that expand another method to regulate on-off state of riboswitch besides the ligand.
