![OPEN PBR LOGO](https://static.igem.org/mediawiki/2019/4/42/T--Humboldt_Berlin--openPBR.png)
Introduction
The openPBR is a low-cost, easy to assemble and reliable option to cultivate phototrophic organisms. We have developed it as an open-source platform to operate as you need it. It comes in several setups, from nine cultivation vessels to three flat panels with a minimum width of 15 mm and up to a volume of 190 ml. It provides users with the ability to measure optical density at wavelengths from 400 nm to 800 nm, online and provides a .csv file output. All parts are openly available and even gas supply is exactly adjustable through a rotameter, depending on the needs of your organism and setup. We want to offer scientists a simple and versatile cultivation platform.
Sensors
Illumination
Gas mixing and pumps
Cultivation chamber
Casing
Overview of single components
![Our Open PBR](https://static.igem.org/mediawiki/2019/3/39/T--Humboldt_Berlin--beschriftet_open_pbr.png)
Chemostat equation
Growth rate and light intensity
OD Measurement
Designing the bioreactor
![Bringing Chlamy to iGEM](https://static.igem.org/mediawiki/2019/b/bb/T--Humboldt_Berlin--reaktor_design.jpeg)
Github Project
Pricing
Additional ressources
This chapter provides you with everything you need to rebuild our setup on your own - including software, a list of used parts and assembly instructions.
[1] Huisman, J., Matthijs, H.C., Visser, P.M. et al. Antonie Van Leeuwenhoek (2002) 81: 117. https://doi.org/10.1023/A:1020537928216
[2] Kliphuis, A. M., Klok, A. J., Martens, D. E., Lamers, P. P., Janssen, M., & Wijffels, R. H. (2012). Metabolic modeling of Chlamydomonas reinhardtii: energy requirements for photoautotrophic growth and maintenance. Journal of applied phycology, 24(2), 253–266. doi:10.1007/s10811-011-9674-3
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