![](https://static.igem.org/mediawiki/2019/3/3c/T--SZU-CHINA--index-04.png)
Micrancide
An RNAi-based herbicide for
Mikania micrantha
Fight GREEN
for More Green
Description
![](https://static.igem.org/mediawiki/2019/b/bd/T--SZU-CHINA--attribution-banner-small.png)
Mikania micrantha Kunth is one of the most destructive invasive species in the world. It was introduced into China in the early 20th century, leading to great destruction of local environment. However, at present, the major approach for controlling Mikania micrantha Kunth is still chemical herbicide spray, which is associated with significant hazards to crops and environment. RNA interference (RNAi) is a conserved mechanism in eukaryotic organisms, in which sequence specific gene silencing is triggered by the introduction of dsRNA. In this project, we used RNAi technology to silence the genes encoding proteins with essential functions to induce mortality in Mikania micrantha Kunth through topical application of E Coli expressed RNAi molecules. In the meantime, we have developed a synthetic kit that can be used to synthesize RNAi nanoparticles of different sizes, and a siRNA sifting program for other researchers to select RNAi molecules for their specific purpose.
![](https://static.igem.org/mediawiki/2019/3/31/T--SZU-CHINA--index2-10.png)
Human Practices
We are calling for everyone to
support the Real Green.
Fight GREEN for More Green .
Experiment
![](https://static.igem.org/mediawiki/2019/f/f3/T--SZU-CHINA--index_logo-11.png)
Design
Come and see how we made possible the manufacturing of Micrancide as well as the Application![](https://static.igem.org/mediawiki/2019/0/0c/T--SZU-CHINA--index_logo-12.png)
Software
Here is the siRNA sifting program for other researchers to select RNAi molecules for their specific purpose.![](https://static.igem.org/mediawiki/2019/4/4e/T--SZU-CHINA--index_logo-13.png)
Iteration
Find out how we decided to use the third generation as the final RNAi-based herbicide![](https://static.igem.org/mediawiki/2019/a/ad/T--SZU-CHINA--index_logo-14.png)