Team:SZU-China/Parts

Part Number	Name	Type	Part Description
BBa_K2912017	R-body_ Caedibacter	Composite	Refractile inclusion bodies
BBa_K2912011	M. micrantha_leaves_ Unigene0029128	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein AB80
BBa_K2912016	G-quadruplexes used to detect miRNA	Basic	a G-quadruplex DNA-based, label-free and ultrasensitive strategy to detect the siRNA

Basic Parts

SZU-China iGEM 2019 team utilizes 14 kinds of new basic parts. Here is their individual function:

1.BBa_K2912000-BBa_K2912003 can encode the protein to assemble the R-body, the self-cracking mechanism.

2.BBa_K2912005-BBa_K2912013 are the different ssDNA sequence to transcribe siRNAs that can silence the gene encoding chlorophyll A-B binding protein of M. micrantha.

3.BBa_K2912014 is Trp attenuator that can act as a suicide switch controlled by the concentration of Tryptophan.

You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K2912000	RebA	Basic	RebA may act as a scaffolding protein to facilitate the major polymerization process
BBa_K2912001	RebB	Basic	The major structural subunit of R bodies
BBa_K2912002	RebC	Basic	RebC may be involved in the modification process of forming the R bodies
BBa_K2912003	RebD	Basic	The role of Reb D in the synthesis and assembly of R bodies has been postulated
BBa_K2912005	M. micrantha_leaves_ Unigene0004558	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein CAP10A
BBa_K2912006	M. micrantha_leaves_ Unigene0004560	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding
BBa_K2912007	M. micrantha_leaves_ Unigene0025307	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein LHCB7
BBa_K2912008	M. micrantha_leaves_ Unigene0026534	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein CAB7
BBa_K2912009	M. micrantha_leaves_ Unigene0028194	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein LHCA3
BBa_K2912010	M. micrantha_leaves_ Unigene0029127	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein AB80
BBa_K2912011	M. micrantha_leaves_ Unigene0029128	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein AB80
BBa_K2912012	M. micrantha_leaves_ Unigene0029129	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein CAB40
BBa_K2912013	M. micrantha_leaves_ Unigene0033458	Basic	It can be transcribed into RNAi molecules that can silence the gene encoding chlorophyll A-B binding family protein LHCA5
BBa_K2912014	Tryptophan attenuator	Basic	Trp attenuator can act as a suicide switch controlled by the concentration of Tryptophan.
BBa_K2912016	G-quadruplexes used to detect miRNA	Basic	a G-quadruplex DNA-based, label-free and ultrasensitive strategy to detect the siRNA

Composite Parts

Self-cracking Mechanism

We combined BBa_K2912000-BBa_K2912003 to form a composite part, which encodes the R-body to be used as the self-cracking mechanism. You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K2912017	R-body_ Caedibacter	Composite	Refractile inclusion bodies

Characterization of
Existing Part

SZU-China team has added quantitative experimental characterization data to an existing Part from the Registry of Standard Biological Parts and documented the experimental characterization on the Part's Main Page on the Registry.

This year, we found that using OD600 to test whether E. coli were killed by Toxin Tse2 was inaccurate and unreliable. Meanwhile, we found a new approach for our project, a G-quadruplex DNA-based, label-free, and ultrasensitive strategy for microRNA detection (click Measurement to see more). To test our new method, we applied the new approach to characterize the existing part. We tested the content of siRNA to reflect the function of Toxin Tse2. You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K314200	Toxin Tse2	Basic	A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracellularly

Improved Part

This year, our team has improved the existing part by adding the tryptophan attenuator. The improved part (in blue) and the original existing part (in green) are presented below. You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K2912015 BBa_K117000	Trp_Lysis gene	Composite	Trp_Lysis gene expression can be controlled by the concentration of Tryptophan