Team:SZU-China/Attributions
Attribution
The SZU-China iGEM 2019 team consists of a group of biologists, bioengineers, and programmers. On this page, you can find out about everyone who has contributed to the project. We want to thank all our advisors and instructors who have assisted us throughout the project, and without whom the project would not have gone so smoothly. We are particularly grateful to all everyone else who has given us inspiration, kind and helpful suggestions, technical and financial assistance and so forth. These contributions have helped us enormously. Unless otherwise specified, all work on this wiki was carried out, and all data collected by us. For a full list of acknowledgments, please see the bottom of this page.
Beixin Mo
Professor Mo is the key person in our project and has given us a lot of professional advice and supports. She is gorgeous and intelligent, who can fundamentally pinpoint the existing problems and propose the corresponding solutions.
Weizhao Chen
Dr. Chen is our mentor, leader of the brainstorm, and sponsor. He organizes students to brainstorm and spare no effort to support the team. He is the sole figure of SZU-China, who is an extremely affable and approachable person and loves football games.
Jiaqi Tang
The leader of the human practices and collaboration with different iGEM teams. He is responsible for team building, team wiki editing, team’s WeChat official account administration, the outlay of the whole team management and deals with many additional works.
Lingling Liao
The leader of the experimental group, responsible for lab works, parts characterization and submission, team wiki editing, and is one of the presentation speakers. She also participates in human practice and collaboration.
Jiantao Mai
Responsible for lab works, parts characterization, team wiki editing and participates in human practice and collaboration.
Yiming Chen
Responsible for shooting the documentary of the whole project, team wiki editing as well as the human practice.
Xinyong Chen
Responsible for designing and developing applet, the Wiki and building servers.
Zijian Li
Responsible for the design of our poster, PPT, WeChat applet and the Wiki ，developing applet and wiki.
Rongsong Ling
Responsible for screening the specific genes from the transcript of Mikania micrantha, making the drone that can identify the weed and spray our RNAi herbicide automatically as well as the modelling works.
Zhongting Deng
Responsible for lab works like phosphorylation of ssDNA, synthesis of circular DNA and production of nanoparticles and participates in human practice and collaboration.
Xiaolong Guo
Responsible for lab works like the quantitation of gene expression and recording apparent phenomena of RNAi herbicide-applying plants. Also participates in human practice and collaboration.
Jingying Lin
Responsible for lab works like testing the function of the RNAi herbicide, including cultivation and extraction of RNA from the leaves of Mikania micrantha and characterization of the gene expression. Also participates in human practice and collaboration.
Yu yu
Ms. Yu is our instructor, who has given us many suggestions on experiment. Her gentle courtesy and quaint speech win everyone’s heart. She is very open-minded and loves to make friends with her students.
Mingyue Luo
Mingyue is our advisor who has participated in 2018 iGEM and has a bunch of experience on how to solve problems during the experiment. He assists us a lot and spares no efforts to help us.
Xiaohan Xie
Xiaohan is our advisor on the Wiki design, as well as the applet, who has offered us valuable experience on how to create the Wiki. She is conscientious, patient and responsible.
Gefei Li
Gefei is our advisor who helps us improve our oral English. She has given many different presentations and has a bunch of experience on how to give a speech.
Yongyi Wang
Yongyi is our advisor who has participated in iGEM before and is skillful in screening target gene. He helps us connect our college’s server and gives us many suggestions on how to analyze the transcript of Mikania micrantha and find the specific gene we need quickly.
Xiaoyan Wang
Dr. Wang is our advisor on the experiment. He has done a lot of researches on RNAi techniques and is skilled at different kinds of molecular experiments, who has provided us many suggestions.
Sun Chao
Dr. Sun is our advisor on the experiment. He has used synthetic dsRNA as an insecticide. He has taught us how to synthesize the dsRNA and solved the problems together with us.
Gene screening
Agricultural Genomics Institute at Shenzhen, CAAS: provided whole genome of Mikania micrantha.
Dr. Bo Song:provided some suggestions on how to better screen the target gene.
Experiment
Professor Shengli Tian: suggested us on how to modify the RNAi nanoparticles.
Dr. Boxiang Wang: suggested us on how to characterize the effects of our RNAi herbicide.
Professor Xuemei Chen: suggested that we should change the ratio of circle DNA to T7 RNA polymerase to create different sizes of RNAi nanoparticles.
Professor Yu Zhang: taught us how to operate the scanning electron microscope and give us some relative tips.
Dr. Bin Jia: suggested us to design a large-scale production line to transcribe the RNAi molecules via E. coli.
Professor Fangfang Xu: taught us something about the E. coli.
Dr. Yeqing Zong: suggested us to use R-Body as the killer switch.
Professor Yong Wang: gave us some advice on the G-quadruplex DNA-based, label-free and ultrasensitive strategy for microRNA detection.
Professor Lijun Zhang: suggested us to use Northern Blot to detect the siRNA inside the plants.
Human Practice
Test field of CAAS, Shenzhen and BGI: provided us with the idea of using agricultural drone to spray our RNAi herbicide.
Fairy lake Botanical Garden: provided us an experimental report on the best herbicide elimination effect of Mikania micrantha at present.
Dr. Shouzhou Zhang: provided information about the invasion history of Mikania micrantha in Shenzhen, including the sites, time, diffusion speed, and the largest area of historical diffusion. He also provided information on the damage of Mikania micrantha to Shenzhen's Green Belt.
Shenzhen Wildlife Conservation and Management Office staff: provided the distribution map and the disaster area of Mikania micrantha in Shenzhen.
Neilingding Island Nature Reserve staff: provided a data base for designing experimental plots for the invasion of Mikania micrantha.
Dr. Pingping Wei: provided a map of Mikania micrantha on Neilingding Island, explained the harm of Mikania micrantha on the island and explained the principle of biological control against Mikania micrantha.
SZU-RobotPilots: provided us some suggestions on how to make the agricultural drone that can identify the Mikania micrantha and spray on the weed automatically.
SZU-Volunteers Lihu campus: helped us to organize the voluntary activity on the elimination of M. micrantha.
Shenzhen Wildlife Protection and Measurement Office:Provided the distribution diagram of M. micrantha in Shenzhen.
Mr. Zhiqiang Xie: Recommended us to use SDK and helped us understand the functions of various parts of drone.
Shenzhen Clear Mikania micrantha Co. Ltd.: Provided us the effect report of their product.
Special thanks: We sincerely thank Jianting Huang, Yaxuan Wang and Minghui Ge, who used to be our team members and had participated in a few parts of project.
Experiment Module
We designed an RNAi-based herbicide for M. micrantha. In order to finish our experiment,, most iGEMers have taken on the heavy responsibility. From screening the specific and essential gene of M. micrantha to testing the RNAi-based herbicide, Rongsong, Lingling, Jiantao, Zhongting, Xiaolong and Jingying have devoted their time and effort. Rongsong designed a siRNA screening program to help us find the specific and essential gene. Lingling designed most of the experiments. Jiantao was responsible for the part characterization and RNAi molecules synthesis. Zhongting was responsible for the RNAi nanoparticles synthesis and characterization. Xiaolong and Jingying were responsible for testing the RNAi-based herbicide. They all made great contributions to the project!
Human Practice Module
In order to build a platform for iGEMers to communicate with each other, Jiaqi and Zhongting organized a meeting which called Southern China Regional Meeting. Besides, Yiming shot a documentary showing the history of M. micrantha invasion. Here, we sincerely thank Jiaqi, Zhongting, Yiming, Jingying, Xiaolong, Jiantao and Lingling, without whom the human practice, as well as the collaboration could not be carried out so successfully.
Modeling
To predict the growth of M. micrantha, the efficiency of our product and help to direct project design, Rongsong constructed many mathematical models and analyzed some experimental data with the statistic model to give us a scientific result.
Wiki & Applet
Our team this year only has two members to build and design the wiki, as well as the Applet. These two members are Xinyong and Zijian. They were the main forces in creating the team wiki and have spent a lot of time to discuss with us and build the wiki.
Instruction
Thanks to our soul figure, Weizhao Chen, we can run our team smoothly. He not only gave us suggestions on various parts of the competition but also faced the problems together with us. More importantly, we could not finish our project without the sponsor he found. Meanwhile, we also thank the key person, Professor Beixin Mo, of our project for giving us a lot of professional advice and supports. Additionally, we thank Yu Yu, our instructor, for telling us many valuable experiences on doing different kinds of experiments.
Sponsors
