| Part Name | Registry Name | Registry Type | Description | Fusion Sites | Position in L1 |
|---|---|---|---|---|---|
| L0 - Backbone | BBa_K2984010 | Plasmid | L0 backbone (plasmid with RFP insert) | - | ▇ |
| L0-AR | BBa_K2984009 | Regulatory | L0 backbone with AR promoter | A1-A3 | ▇ |
| L0-AR | BBa_K2984025 | Regulatory | L0 backbone with AR promoter | A1-B1 | ▇ |
| L0-PsaD | BBa_K2984022 | Regulatory | L0 backbone with PsaD promoter | A1-A3 | ▇ |
| L0-PsaD | BBa_K2984008 | Regulatory | L0 backbone with PsaD promoter | A1-B1 | ▇ |
| L0-ARS1 | BBa_K2984000 | Coding | L0 backbone with 3'UTR Arylsulfatase1 secretion signal | B2-B2 | - |
| L0-GLE | BBa_K2984001 | Coding | L0 backbone with 3'UTR gametolysin secretion signal | B2-B2 | - |
| L0-PETase | BBa_K2984011 | Coding | L0 backbone with PETase CDS | B3-B4 | - |
| L0-Hyg | BBa_K2984012 | Coding | L0 backbone with Hygromycin B resistance CDS | B3-B4 | - |
| L0-Paro | BBa_K2984006 | Coding | L0 backbone with Paromomycine resistance CDS | B3-B4 | - |
| L0-ptxD | BBa_K2984026 | Coding | L0 backbone with phosphite oxidoreductase CDS | B3-B3 | - |
| L0-ptxD | BBa_K2984013 | Coding | L0 backbone with phosphite oxidoreductase CDS | B3-B4 | - |
| L0-YFP | BBa_K2984017 | Coding | L0 backbone with yellow fluorescent protein CDS | B3-B4 | - |
| L0-YFP | BBa_K2984024 | Coding | L0 backbone with yellow fluorescent protein CDS | B4-B4 | - |
| L0-YFP | BBa_K2984020 | Coding | L0 backbone with yellow fluorescent protein CDS | B5-B5 | - |
| L0-6xHis tag | BBa_K2984014 | Tag | L0 backbone with 6x histidine tag | B5-B5 | - |
| L0-3xHis tag | BBa_K2984015 | Tag | L0 backbone with 3x histidine tag | B5-B5 | - |
| L0-SP20 | BBa_K2984016 | Coding | L0 backbone with glycomodule for secretion enhancement | B5-B5 | - |
| L0-RbcS2 | BBa_K2984018 | Terminator | L0 backbone with RuBisCo terminator | B5-C1 | - |
| L0-RbcS2 | BBa_K2984021 | Terminator | L0 backbone with RuBisCo terminator | B6-C1 | - |
| L0-Linker | BBa_K2984034 | Intermediate | L0 backbone with Linker sequence | B2-B2 | - |
| L0-Ble | BBa_K2984040 | Coding | L0 backbone with Bleomycin resistance | B1-B1 | - |
| L0-scp | BBa_K2984044 | Intermediate | L0 backbone with Bleomycin resistance | B1-B1 | - |
| L0-PsaDIntron | BBa_K2984046 | Regulatory | L0 backbone with PsaDIntron | - | - |
| L0-PETase | BBa_K2984049 | Regulatory | L0 backbone with PETase | B3-B3 | - |
| L0-RFP | BBa_K2984004 | Coding | L0 backbone with RFP | - | - |
| L0-amp | BBa_K2984007 | Coding | L0 backbone with ampilicin resistance | - | - |
| L0-amp | BBa_K29858 | Intermediate | Linker L1c -> L0 | - | - |
| L0 Linker | BBa_K29860 | Intermediate | Linker L0 -> L1a | - | - |
| L0 Linker | BBa_K29861 | Intermediate | Linker L0 -> L1b | - | - |
| Part Name | Registry Name | Registry Type | Description |
|---|---|---|---|
| L1a | BBa_K2984003 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| L1b | BBa_K2984005 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| L1c | BBa_K2984002 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| L2 | BBa_K29862 | Plasmid Backbone | Level 2 backbone with paromycin resistance flanked by GoldenGate restiction site |
| Part Name | Registry Name | Registry Type | Description |
|---|---|---|---|
| L1-PsaD-Paro-RbcS2 | BBa_K2984055 | Coding | Construct lending paromomycin resistance to C. reinhardtii |
| L1-PsaD-Hyg-RbcS2 | BBa_K2984056 | Coding | Construct lending hygromycin resistance to C. reinhardtii |
| L1-PsaD-ARS-PETase-YFP-SP20-RbcS2 | BBa_K2984028 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1-PsaD-GLE-PETase-YFP-SP20-RbcS2 | BBa_K2984029 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1-PsaD-ARS-PETase-YFP-RbcS2 | BBa_K2984031 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1-PsaD-ARS-PETase-3xHA-RbcS2 | BBa_K2984032 | Coding | Secretion of PETase and purification via HA-tag |
| L1c-PsaD-YFP-RbcS2 | BBa_K2984019 | Coding | Reference for fluorescence measurements |
| L1c-PsaD-ARS-YFP-Rbsc2 | BBa_K2984027 | Coding | Secretion of YFP |
| L1c-PsaD-ARS-YFP-SP20-Rbcs2 | BBa_K2984030 | Coding | Secretion of YFP to test the ARS secretion signal and SP20 efficiency |
| L1c-PsaD-PETase-YFP-Rbcs2 | BBa_K2984036 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1c-AR-ARS-YFP-Rbcs2 | BBa_K2984033 | Coding | Secretion of YFP |
| L1c-PsaD-PETase-YFP-His-RbcS2 | BBa_K2984035 | Coding | Expression of PETase and expression measurement via YFP fluorescence to be purified via His-Tag |
| L1c-PsaD-PtxD-Rbsc2 | BBa_K2984037 | Coding | Evaluation of C. reinhardtii growth on phosphite-containing media |
| L1c-Psad-PETase-ptxD-Rbcs2 | BBa_K2984038 | Coding | Expression of PETase and evaluation ofC. reinhardtiigrowth on phosphite-containing media |
| L1c-PsaD-PETase-3xHA-RBCS2 | BBa_K2984039 | Coding | Expression of PETase and purification via HA-Tag |
| L1c-PsaD-ARS-PETase-3xHA-SP20-RbcS2 | BBa_K2984041 | Coding | Enhanced Secretion of PETase and SP20 efficiency and purification via HA-Tag |
| L1c-PsaD-MHETase-YFP-RbcS2 | BBa_K2984042 | Coding | Expression of MHETase and expression measurement via YFP fluorescence |
| L1c-PsaD-MHETase-3xHA-RbcS2 | BBa_K2984043 | Coding | Expression of MHETase and purification via HA-Tag |
| bleR+scp | BBa_K2984045 | Coding | Bleomycin resistance fused to self cleaving peptide |
| L1c-AR-PETase-YFP-RbcS2 | BBa_K2984047 | Coding | Expression of PETase and expression measurements via YFP fluorescence |
Here's how our color-code works
| Promoter | 5' UTR | CDS | CDS/Tag | Terminator |
|---|---|---|---|---|
| A1/A3A1/B1 | B1/B2 | B3/B4B3/B3 | B4/B4 | B5/C1(B6/C1) |
| ▇ | ▇ | ▇ | ▇ | ▇ |
Go to top