| Part Name | Registry Name | Registry Type | Description | Fusion Sites | Position in L1 |
|---|---|---|---|---|---|
| L0 - Backbone | BBa_K2984010 | Plasmid | L0 backbone (plasmid with RFP insert) | - | ▇ |
| L0-AR | BBa_K2984009 | Regulatory | L0 backbone with AR promoter | A1-A3 | ▇ |
| L0-AR | BBa_K2984025 | Regulatory | L0 backbone with AR promoter | A1-B1 | ▇ |
| L0-PsaD | BBa_K2984022 | Regulatory | L0 backbone with PsaD promoter | A1-A3 | ▇ |
| L0-PsaD | BBa_K2984008 | Regulatory | L0 backbone with PsaD promoter | A1-B1 | ▇ |
| L0-ARS1 | BBa_K2984000 | Coding | L0 backbone with 3'UTR Arylsulfatase1 secretion signal | B2-B2 | - |
| L0-GLE | BBa_K2984001 | Coding | L0 backbone with 3'UTR gametolysin secretion signal | B2-B2 | - |
| L0-PETase | BBa_K2984011 | Coding | L0 backbone with PETase CDS | B3-B4 | - |
| L0-Hyg | BBa_K2984012 | Coding | L0 backbone with Hygromycin B resistance CDS | B3-B4 | - |
| L0-Paro | BBa_K2984006 | Coding | L0 backbone with Paromomycine resistance CDS | B3-B4 | - |
| L0-ptxD | BBa_K2984026 | Coding | L0 backbone with phosphite oxidoreductase CDS | B3-B3 | - |
| L0-ptxD | BBa_K2984013 | Coding | L0 backbone with phosphite oxidoreductase CDS | B3-B4 | - |
| L0-YFP | BBa_K2984017 | Coding | L0 backbone with yellow fluorescent protein CDS | B3-B4 | - |
| L0-YFP | BBa_K2984024 | Coding | L0 backbone with yellow fluorescent protein CDS | B4-B4 | - |
| L0-YFP | BBa_K2984020 | Coding | L0 backbone with yellow fluorescent protein CDS | B5-B5 | - |
| L0-6xHis tag | BBa_K2984014 | Tag | L0 backbone with 6x histidine tag | B5-B5 | - |
| L0-3xHis tag | BBa_K2984015 | Tag | L0 backbone with 3x histidine tag | B5-B5 | - |
| L0-SP20 | BBa_K2984016 | Coding | L0 backbone with glycomodule for secretion enhancement | B5-B5 | - |
| L0-RbcS2 | BBa_K2984018 | Terminator | L0 backbone with RuBisCo terminator | B5-C1 | - |
| Part Name | Registry Name | Registry Type | Description |
|---|---|---|---|
| L1a | BBa_K2984003 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| L1b | BBa_K2984005 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| L1c | BBa_K2984002 | Plasmid backbone | Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site |
| Part Name | Registry Name | Registry Type | Description |
|---|---|---|---|
| L1-PsaD-Paro-RbcS2 | BBa_K2984055 | Coding | Construct lending paromomycin resistance to C. reinhardtii |
| L1-PsaD-Hyg-RbcS2 | BBa_K2984056 | Coding | Construct lending hygromycin resistance to C. reinhardtii |
| L1-PsaD-ARS-PETase-YFP-SP20-RbcS2 | BBa_K2984028 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1-PsaD-GLE-PETase-YFP-SP20-RbcS2 | BBa_K2984029 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |
| L1-PsaD-ARS-PETase-YFP-RbcS2 | BBa_K2984031 | Coding | Secretion of PETase and expression measurement via YFP fluorescence |