Team:Humboldt Berlin/Contribution

It's colorfull

Characterizing Chromoproteins

We encountered problems when measuring the the YFP-signal in our transformed C. rheinhardtii YFP strains, even though we were able to detect YFP when examining the cells with the fluorescence microscope. To get a better understanding of the protocols followed to measure absorbance and fluorescence spectra with a plate reader, we conducted some exemplary measurements with fluorescent and non-fluorescent chromoproteins expressed in E. coli. We tested three different chromoproteins from the iGEM Registry of Standard Biological Parts in terms of their absorption spectra (as well as the fluorescence spectrum in case of amajLime):

amajLime from the coral Anemonia majato BBa_K1033914
spisPink from the coral Stylophora pistillata BBa_K1033923
gfasPurple from the coral Galaxea fascicularis BBa_K1033917

Therefore we transformed the pSB1C3 vector with the respective chromoprotein constructs (which all carry a promotor and RBS sequence) into E. coli (DH10B Competent Cells). After cultivation and we lysed the harvested cells according to our lysis protocol .