Team:Humboldt Berlin/Results

Synthesis of L0 and L1 gene constructs

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Expression of YFP-containing constructs

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Fig. - Fluorescence intensity of C. reinhardtii WT and a YFP carrying clone, in decreasing optical density of the cell culture. Excitation at 490 nm and emission measurement at 528 nm. The results clearly show that the fluorescence of the YFP expressing clone is higher than the autofluorescence of the WT algae. fluorescence difference spectrum

Fig. - YFP emission spectrum of a C. reinhardtii clone with YFP with an emission maximum at approximately 530 nm. Difference spectrum of WT and YFP spectra fluorescence intensity time resolved

Fig. - Time resolved measurement of the YFP fluorescence intensity. Light induction of the PsaD promoter (white area). It can clearly be seen that at the time of illumination the fluorescence intensity of YFP increases, indicating a light induced expression of the protein.

Secretion of Enzymes

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PtxD-controlled growth

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PET-degradation in-silico

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PET degradation by C. reinhardtii

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Growth experiments

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