Team:Humboldt Berlin/Attributions

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Attributions

Attributions

ideonella grafic

Supporters

Our team accomplished great things in the project Chlamylicious over the course of this year’s iGEM competition: We adapted a toolkit for the green algae C. reinhardtii with parts for successful and easy cloning of any transcription unit, which we implemented for the expression of the PET-degrading enzyme PETase by the algae and designed a bioreactor for its cultivation under constant monitoring of all parameters. These were our greatest supporters:

  • our host laboratories Experimental Biophysics and Molecular Genetics from the Humboldt University of Berlin, who shared their infrastructure with us

  • our supervisor Prof. Dr. Schmitz-Linneweber
  • the principal investigator Simon Kelterborn, who guided us with his expertise in transformations of Chlamydomonas and CRISPR-aided genetic engineering

  • the biological technical assistants Heide (Biophysics lab), Conny, Thea and Irina (all Genetics lab), who enabled us to launch the wet-lab

  • computer scientist Vincent Will, who implemented the design for our website

  • electrical engineer Markus Schultz, who helped designing and wiring the electronics for our bioreactor

  • technical engineering student Alejandro Gómez Jimenez from the Technical University of Berlin, who wrote the CAD-models for all parts of our bioreactor

  • the Interdisciplinary Workshop „Matters of Activity“ of Humboldt University, who laser-cut our hardware

  • the Mechanical Workshop of the Physics Department of our university

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Timeline and responsibilities

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Pverview of the hierarchical and modular cloning system

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