Scientific Collaborations
iGEM Marburg Link
We tied first bonds with the Marburg iGEM-Team at the German Meet-Up in Düsseldorf and soon decided to start a collaboration. We took part in their Interlab-Study testing a standardized protocol for Golden Gate-reactions. As the MoClo-Toolkit we are working with is also based on Golden Gate reactions this was a splendid opportunity to generate data, validate an improved protocol and show the possibilities and especially the benefits of the Golden Gate method. Our gathered data shows that the improved protocol works as reliably as the long one (see table and pictures below). The reactions were pipetted following their Interlab Study guide book: Interlab Protocol_final.pdf Additionally we decided to cultivate Marburg`s Synechococcus strains to improve our knowledge on the needs of phototrophic organisms and to gather more reliable data on their growth rate and growth conditions.
| Protocol | Amount Plated (µl) | Green Colonies | Red Colonies | White Colonies | Comments |
|---|---|---|---|---|---|
| Troubleshoot | 100 | 228 | 0 | 0 | dh5alpha, BsaI |
| Troubleshoot | 100 | 57 | 0 | 0 | dh5alpha, BsaI |
| Troubleshoot | 100 | 139 | 0 | 0 | dh5alpha, BsaI |
| Rapid | 100 | 5 | 0 | 0 | dh5alpha, BsaI |
| Rapid | 100 | 20 | 0 | 0 | dh5alpha, BsaI |
| Rapid | 100 | 31 | 0 | 0 | dh5alpha, BsaI |
| Improved | 100 | 23 | 0 | 0 | dh5alpha, BsaI |
| Improved | 100 | 35 | 0 | 0 | dh5alpha, BsaI |
| Improved | 100 | 224 | 0 | 0 | dh5alpha, BsaI |
iGEM Kaiserslautern Link
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iGEM Dresden Link
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Outreach Collaborations
iGEM Düsseldorf
The overall aim of iGEM is to not only design and run a project but to engage and sensitize the public with synthetic biology. This is why we collaborated with iGEM Düsseldorf and their postcard project. Former iGEM teams of the Heinrich-Heine-University Düsseldorf established the project by collecting self-designed postcards from iGEM-teams all over the world. Every team had to create a personal postcard with an image and a short informational text related to their topic. iGEM Düsseldorf collected all of them and sended those collections back to every team that participated. We then received iGEM postcards from all over the world, which showed us the huge variety and diversity of the iGEM competition. Most importantly it gave us the possibility to share those postcards whenever we organized events about synthetic biology, such as the panel discussion or The Long Night Of The Sciences in Berlin.
iGEM Freiburg: NietorP
As a part of our project was dedicated to sustainability and the battle against man-made problems we were happy to participate in iGEM-Freiburgs challenge which requested teams to take actions for a better environment: Collecting trash, swapping public-transport for bike and sharing pictures of this team-experience. It was a fun way to think about how simple changes in lifestyle could have a huge impact for the preservation of our earth. And by posting this on instagram we shared this vision with the world.
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