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| <p class="medium-sized"></h3> | | <p class="medium-sized"></h3> |
− | The sensory system of the openPBR consist of up to nine Opt 101 Monolithic linear voltage sensors and nine LEDs with the specific wavelength of 680 nm. It is possible to use any LED in the linear area of the sensor thus measurements from 400 nm to 800 nm are feasible. | + | The sensory system of the openPBR consists of up to nine Opt 101 Monolithic linear voltage sensors and nine LEDs with the specific wavelength of 680 nm. It is possible to use any LED in the linear area of the sensor thus measurements from 400 nm to 800 nm are feasible. |
| </p> | | </p> |
| <img src="https://static.igem.org/mediawiki/2019/0/0d/T--Humboldt_Berlin--opt.jpg" alt="optical responsivity" /> | | <img src="https://static.igem.org/mediawiki/2019/0/0d/T--Humboldt_Berlin--opt.jpg" alt="optical responsivity" /> |
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− | The pumps of the openPBR are versatile in their functions. They can be used to pump in and out media or to connect multiple chambers, for example to dilute the cultures. To control the cell density in the cultivation chamber, the openPBR measures the cell density and indicates the pump system to keep turbidostatic conditions. On a given density one pump pumps out media/cell culture and the second pump pumps in new media. | + | The pumps of the openPBR are versatile in their functions. They can be used to pump media in and out or to connect multiple chambers, for example to dilute the cultures. To control the cell density in the cultivation chamber, the openPBR measures the cell density and indicates the pump system to keep turbidostatic conditions. On a given density one pump pumps out media/cell culture and the second pump pumps in new media. |
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| </p> | | </p> |
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| <div> | | <div> |
| <p> | | <p> |
− | <a href="/Team:Humboldt_Berlin/Model#chmstt"> The chemostat equation </a> which we treated as a part of modeling provides us with an overview of general parameters important to a microbial culture. | + | <a href="/Team:Humboldt_Berlin/Model#chmstt"> The chemostat equation </a> which we treated as a part of modelling provides us with an overview of general parameters important to a microbial culture. |
| If we want to cultivate in turbidostat-mode, we need to remove liquid from the culture continuously. This removing is quantified through dilution rate \( D \): | | If we want to cultivate in turbidostat-mode, we need to remove liquid from the culture continuously. This removing is quantified through dilution rate \( D \): |
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| \end{array} | | \end{array} |
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− | This equation allows allows to quantify dilution of the culture independently of cultivation setup. With characteristic values between \( 0.018 - 0.064 \, \frac{1}{h} \), we can calculate the rate at which our pumps should pump medium out of the culture: | + | This equation allows us to quantify dilution of the culture independently of the cultivation setup. With characteristic values between \( 0.018 - 0.064 \, \frac{1}{h} \), we can calculate the rate at which our pumps should pump medium out of the culture: |
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| \begin{equation} D \cdot V_{c} = \frac{\mathrm{d}}{\mathrm{d}t} V_{in} \end{equation} | | \begin{equation} D \cdot V_{c} = \frac{\mathrm{d}}{\mathrm{d}t} V_{in} \end{equation} |
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| <p class="medium-sized"></h3> | | <p class="medium-sized"></h3> |
− | Since we are a non-profit team of our own, we know that financing is one of the limiting and most problematic challenges of this contest. So it was important to us, that every iGEM team who wants to continue our project ,or any new project using a phototrophic organism, has a low-price but efficient way to fulfil their great ideas. For only 588 € it is possible to rebuild our bioreactor that offers many opportunities to design a set-up tailored to the needs of innovative projects. | + | Since we are a non-profit team of our own, we know that financing is one of the limiting and most problematic challenges of this contest. So it was important to us, that every iGEM team who wants to continue our project ,or any new project using a phototrophic organism, has a low-price but efficient way to fulfill their great ideas. For only 588 € it is possible to rebuild our bioreactor that offers many opportunities to design a set-up tailored to the needs of innovative projects. |
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| </p> | | </p> |