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| </div> | | </div> |
| <div> | | <div> |
− | | + | <figure> |
| + | <img class="is-revealing" |
| + | img src="https://static.igem.org/mediawiki/2019/f/f9/T--Humboldt_Berlin--exhibition_space.jpeg"/><figcaption> <b>Presenting the openPBR at the Giant Jamboree 2019.</b> For three days many teams, judges and unaffiliated guests of the Giant Jamboree discussed with us the utility and advantages of the a modular cultivation setup. The feedback will influence our further design iterations.</figcaption> |
| + | </figure> |
| </div> | | </div> |
| </div> | | </div> |
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| <div> | | <div> |
| <!-----------------------------------------------------------------------> | | <!-----------------------------------------------------------------------> |
− | <!------------------PET degradation-----------------------> | + | <!------------------Overview components-----------------------> |
| <!-----------------------------------------------------------------------> | | <!-----------------------------------------------------------------------> |
| <UL class="no-bulletpoint"> | | <UL class="no-bulletpoint"> |
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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/d/d0/T--Humboldt_Berlin--Ligth_Intensity_Heat_15.png" alt="Heatmap 1" /><figcaption><b>Fig. 5 </b>Light intensity distribution inside the opbenPBR for relative voltage setting of 15</figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/d/d0/T--Humboldt_Berlin--Ligth_Intensity_Heat_15.png" alt="Heatmap 1" /><figcaption><b>Fig. 5</b> Light intensity distribution inside the opbenPBR for the relative voltage setting of 15.</figcaption></figure> |
| </div> | | </div> |
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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/d/dc/T--Humboldt_Berlin--Ligth_Intensity_Heat140.png" alt="Heatmap 2" /><figcaption><b>Fig.6</b> Light intensity distribution inside the opbenPBR for relative voltage setting of 140 </figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/d/dc/T--Humboldt_Berlin--Ligth_Intensity_Heat140.png" alt="Heatmap 2" /><figcaption><b>Fig .6</b> Light intensity distribution inside the opbenPBR for the relative voltage setting of 140. </figcaption></figure> |
| </div> | | </div> |
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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/b/bf/T--Humboldt_Berlin--Ligth_Intensity_Heat225.png" alt="Heatmap 3" /><figcaption><b>Fig.7</b> Light intensity distribution inside the opbenPBR for relative voltage setting of 225 </figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/b/bf/T--Humboldt_Berlin--Ligth_Intensity_Heat225.png" alt="Heatmap 3" /><figcaption><b>Fig. 7</b> Light intensity distribution inside the opbenPBR for the relative voltage setting of 225. </figcaption></figure> |
| </div> | | </div> |
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| <img src="https://static.igem.org/mediawiki/2019/0/0f/T--Humboldt_Berlin--leistebro.jpg" alt="sensor rail" /> | | <img src="https://static.igem.org/mediawiki/2019/0/0f/T--Humboldt_Berlin--leistebro.jpg" alt="sensor rail" /> |
| <br /> | | <br /> |
− | <figcaption> <b>Fig. 9. Rail containing the light sensors. </b> The rail is attached to the front of the openPBR and detects the light that passes through the culture.</figcaption> | + | <figcaption> <b>Fig. 9. Rail containing the light sensors. </b>The rail is attached to the front of the openPBR and detects the light that passes through the culture.</figcaption> |
| </div> | | </div> |
| <div> | | <div> |
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− | <h3 class="headline3" style="font-size: 30px; margin-left: 80px"> Callibration</h3> | + | <h3 class="headline3" style="font-size: 30px; margin-left: 80px"> Calibration</h3> |
| | | |
| <div class="two-columns block-text medium-sized not-centered no-margin-top" style="margin-bottom:0px"> | | <div class="two-columns block-text medium-sized not-centered no-margin-top" style="margin-bottom:0px"> |
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| <p> | | <p> |
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− | Once the sensors are installed, you need to callibrate them. In order to get a net voltage, you need to substract the output value from the input value. Subtract the Vout values from the Vin values and plot them against the reference OD from your photometer. Then perform a logarithmic fit (Fig. 10) | + | Once the sensors are installed, you need to calibrate them. To get a net voltage, you need to subtract the output value from the input value. Subtract the V<sub>out</sub> values from the V<sub>in</sub> values and plot them against the reference OD of your photometer. Then perform a logarithmic fit as shown in Fig. 10. |
| </p> | | </p> |
| <figure> | | <figure> |
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| <figcaption><b>Fig.10:</b> Measured OD with reference photometer against measured ∆V with openPBR </figcaption> </figure> | | <figcaption><b>Fig.10:</b> Measured OD with reference photometer against measured ∆V with openPBR </figcaption> </figure> |
| | | |
− | After the calibration, we measured other random samples both with our reference photometer (Expected OD) and with openPBR (Figure 2). | + | After calibration, we measured other random samples both with our reference photometer (Expected OD) and with the openPBR (Figure 2). |
− | We also plotted the “ideal correlation”, meaning expected = measured, in order to get an idea of how good the openPBR measurements are. | + | We also plotted the “ideal correlation”, meaning expected value = measured value, in order to get an idea of how good the openPBR measurements were. |
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| </div> | | </div> |
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| <figure> | | <figure> |
| <img src="https://static.igem.org/mediawiki/2019/7/7b/T--Humboldt_Berlin--ExpectedOD.png"/> | | <img src="https://static.igem.org/mediawiki/2019/7/7b/T--Humboldt_Berlin--ExpectedOD.png"/> |
− | <figcaption><b>Fig.11:</b> Expected OD values from reference photometer plotted against openPBR measurements with an “ideal correlation” of x = y </figcaption> </figure> | + | <figcaption><b>Fig.11:</b> Expected OD values of the reference photometer plotted against openPBR measurements with an “ideal correlation” of x = y </figcaption> </figure> |
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| <p> | | <p> |
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| <p class="medium-sized"></h3> | | <p class="medium-sized"></h3> |
− | The casing of the openPBR consists of 10 unique laser-cut Plexiglas parts. The whole openPBR can be assembled without the use of screws. Adjustment of the cultivation temperature can be achieved by regulating the water temperature of the filled inner chamber of the openPBR. | + | The casing of the openPBR consists of 10 unique laser-cut plexiglas parts. The whole openPBR can be assembled without making use of screws. Adjustment of the cultivation temperature is achieved by regulating the water temperature of the filled inner chamber of the openPBR. |
| <br> | | <br> |
| The peristaltic pumps are held by mounts fitted to the model so that they are kept in place solely by friction. All the laser cutting files and tutorials are available on GitHub. | | The peristaltic pumps are held by mounts fitted to the model so that they are kept in place solely by friction. All the laser cutting files and tutorials are available on GitHub. |
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| <img src="https://static.igem.org/mediawiki/2019/7/74/T--Humboldt_Berlin--lasercut_file.PNG" alt="Overview of the hierarchical and modular cloning system" /> | | <img src="https://static.igem.org/mediawiki/2019/7/74/T--Humboldt_Berlin--lasercut_file.PNG" alt="Overview of the hierarchical and modular cloning system" /> |
| | | |
− | <figcaption> <b>Fig. 12. Overview of the lasercut file.</b> Files for laser-cutting Plexiglas to build the casing are online on our <a href="https://github.com/igemsoftware2019/Humboldt_Berlin">GitHub page</a>.</figcaption> | + | <figcaption> <b>Fig. 12. Overview of the laser cut file.</b> Files for laser-cutting plexiglas to build the casing are online on our <a href="https://github.com/igemsoftware2019/Humboldt_Berlin">GitHub page</a>.</figcaption> |
| </div> | | </div> |
| <div> | | <div> |