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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/d/d0/T--Humboldt_Berlin--Ligth_Intensity_Heat_15.png" alt="Heatmap 1" /><figcaption>Fig. 5 Light intensity distribution inside the opbenPBR fpr relative voltage setting of 15</figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/d/d0/T--Humboldt_Berlin--Ligth_Intensity_Heat_15.png" alt="Heatmap 1" /><figcaption><b>Fig. 5 </b>Light intensity distribution inside the opbenPBR for relative voltage setting of 15</figcaption></figure> |
| </div> | | </div> |
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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/d/dc/T--Humboldt_Berlin--Ligth_Intensity_Heat140.png" alt="Heatmap 2" /><figcaption>Fig.6 Light intensity distribution inside the opbenPBR fpr relative voltage setting of 140 </figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/d/dc/T--Humboldt_Berlin--Ligth_Intensity_Heat140.png" alt="Heatmap 2" /><figcaption><b>Fig.6</b> Light intensity distribution inside the opbenPBR for relative voltage setting of 140 </figcaption></figure> |
| </div> | | </div> |
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| <div style=" float: left; width: 33.3%; padding: 5px;"> | | <div style=" float: left; width: 33.3%; padding: 5px;"> |
− | <figure><img src="https://static.igem.org/mediawiki/2019/b/bf/T--Humboldt_Berlin--Ligth_Intensity_Heat225.png" alt="Heatmap 3" /><figcaption>Fig.7 Light intensity distribution inside the opbenPBR fpr relative voltage setting of 225 </figcaption></figure> | + | <figure><img src="https://static.igem.org/mediawiki/2019/b/bf/T--Humboldt_Berlin--Ligth_Intensity_Heat225.png" alt="Heatmap 3" /><figcaption><b>Fig.7</b> Light intensity distribution inside the opbenPBR for relative voltage setting of 225 </figcaption></figure> |
| </div> | | </div> |
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| <img src="https://static.igem.org/mediawiki/2019/8/8e/T--Humboldt_Berlin--gasbro.jpg" alt="Our Gas and mixing pumps" /> | | <img src="https://static.igem.org/mediawiki/2019/8/8e/T--Humboldt_Berlin--gasbro.jpg" alt="Our Gas and mixing pumps" /> |
| <br /> | | <br /> |
− | <figcaption> <b>Fig. 8. Assembled Schott-bottle with gas supply pump. </b></figcaption> | + | <figcaption> <b>Fig. 8. Assembled Schott-bottle with gas supply pump.</b> Gas supply is controlled by a rotameter</figcaption> |
| </div> | | </div> |
| <div> | | <div> |
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| <figure> | | <figure> |
| <img src="https://static.igem.org/mediawiki/2019/e/e7/T--Humboldt_Berlin--CallibrationData.png" /> | | <img src="https://static.igem.org/mediawiki/2019/e/e7/T--Humboldt_Berlin--CallibrationData.png" /> |
− | <figcaption>Fig.10: Measured OD with reference photometer against measured ∆V with openPBR </figcaption> </figure> | + | <figcaption><b>Fig.10:</b> Measured OD with reference photometer against measured ∆V with openPBR </figcaption> </figure> |
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| After the calibration, we measured other random samples both with our reference photometer (Expected OD) and with openPBR (Figure 2). | | After the calibration, we measured other random samples both with our reference photometer (Expected OD) and with openPBR (Figure 2). |
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| <figure> | | <figure> |
| <img src="https://static.igem.org/mediawiki/2019/7/7b/T--Humboldt_Berlin--ExpectedOD.png"/> | | <img src="https://static.igem.org/mediawiki/2019/7/7b/T--Humboldt_Berlin--ExpectedOD.png"/> |
− | <figcaption>Fig.11: Expected OD values from reference photometer plotted against openPBR measurements with an “ideal correlation” of x = y </figcaption> </figure> | + | <figcaption><b>Fig.11:</b> Expected OD values from reference photometer plotted against openPBR measurements with an “ideal correlation” of x = y </figcaption> </figure> |
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| <p> | | <p> |
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| <img src="https://static.igem.org/mediawiki/2019/7/74/T--Humboldt_Berlin--lasercut_file.PNG" alt="Overview of the hierarchical and modular cloning system" /> | | <img src="https://static.igem.org/mediawiki/2019/7/74/T--Humboldt_Berlin--lasercut_file.PNG" alt="Overview of the hierarchical and modular cloning system" /> |
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− | <figcaption> <b>Fig. 12. Overview of the lasercut file.</b>Files for laser-cutting Plexiglas to build the casing are online on our <a href="https://github.com/igemsoftware2019/Humboldt_Berlin">GitHub page</a>.</figcaption> | + | <figcaption> <b>Fig. 12. Overview of the lasercut file.</b> Files for laser-cutting Plexiglas to build the casing are online on our <a href="https://github.com/igemsoftware2019/Humboldt_Berlin">GitHub page</a>.</figcaption> |
| </div> | | </div> |
| <div> | | <div> |
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| <div> | | <div> |
| <img src="https://static.igem.org/mediawiki/2019/0/0d/T--Humboldt_Berlin--connectedbro.jpg" /> | | <img src="https://static.igem.org/mediawiki/2019/0/0d/T--Humboldt_Berlin--connectedbro.jpg" /> |
− | <figcaption> <b>Fig. 14. Pumps for turbidostat conditions.</b>To maintain the optical density of a culture, this is the needed setup of connected pumps to pump in fresh media, while pumping out cells.</figcaption> | + | <figcaption> <b>Fig. 14. Pumps for turbidostat conditions.</b> To maintain the optical density of a culture, this is the needed setup of connected pumps to pump in fresh media, while pumping out cells.</figcaption> |
| </div> | | </div> |
| </div> | | </div> |
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| <div> | | <div> |
| <figure> | | <figure> |
− | <img src="https://static.igem.org/mediawiki/2019/d/db/T--Humboldt_Berlin--strombro.jpg" /> | + | <img src="https://static.igem.org/mediawiki/2019/d/db/T--Humboldt_Berlin--strombro.jpg" /><figcaption><b>Fig. 16.</b> Assembled electronics for the cultivation set-up. |
| + | </figcaption> |
| </figure> | | </figure> |
| </div> | | </div> |
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| <figure> | | <figure> |
| <img src="https://static.igem.org/mediawiki/2019/f/f3/T--Humboldt_Berlin--laser_measurement.jpg" alt="Laser measuring OD" /> | | <img src="https://static.igem.org/mediawiki/2019/f/f3/T--Humboldt_Berlin--laser_measurement.jpg" alt="Laser measuring OD" /> |
− | <figcaption>Fig. 16. Universal MoClo fusion sites. | + | <figcaption><b>Fig. 17. Laser measuring optical density.</b> Sensors are mounted on the aluminium rail at the reactor front. |
| </figcaption> </figure> | | </figcaption> </figure> |
| </div> | | </div> |