Introduction
The openPBR is a low-cost, easy to assemble and reliable option to cultivate phototrophic organisms. We have developed it as an open-source platform to operate as you need it. It comes in several setups, from nine cultivation vessels to three flat panels with a minimum width of 15 mm and up to a volume of 190 ml. It provides users with the ability to measure optical density at wavelengths from 400 nm to 800 nm, online and provides a .csv file output. All parts are openly available and even gas supply is exactly adjustable through a rotameter, depending on the needs of your organism and setup. We want to offer scientists a simple and versatile cultivation platform.
Sensors
Illumination
Gas mixing and pumps
Cultivation chamber
Casing
Overview of single components
Chemostat equation
Light Gradient
OD Measurement
Designing the bioreactor
Github Project
Pricing
Additional ressources
This chapter provides you with everything you need to rebuild our setup on your own - including software, a list of used parts and assembly instructions.
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