Difference between revisions of "Team:ECUST China/Notebook"

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{{ECUST_China}}
 
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<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:ECUST_China/ecustbox.css&action=raw&ctype=text/css"/> 
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<link href="https://2019.igem.org/Template:ECUST_China/font.css" rel="stylesheet">
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<body>
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<div id="ecust-box" > 
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<div class="img-main-box">
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<img class="img-main" src="https://static.igem.org/mediawiki/2019/b/be/T--ECUST_China--project_main.png"/>
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      <img class="img-mask" src="https://static.igem.org/mediawiki/2019/5/5a/T--ECUST_China--notebok_mask.png">
  
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      <div class="mininav-left">
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<ul class="circle">
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<li>01
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<a class="mininav-item"  href="#main1" style="padding-top: 15px;">Introduction</a>
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</li>
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<li>02
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<a class="mininav-item"  href="#main2" style="padding-bottom: 15px;">Progress</a>
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</li>
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</ul>
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</div>
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<div class="mian-text-box">
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  <div class="attri-box">
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      <h1 id="main1">Introduction</h1>
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      <p>Paper Transformer is a superhero we made to change the paper world, and several of team members devoted most of their time and energy this season.</p>
 +
      <p>We assigned four iGEMers to achieve three abilities of our Paper Transformer. In detail, we assigned Hu Shijia for cellulose degrading module, Xiang Lan for cellulose producing module, Miao Zongjie and Jin Qianwei for inverter module.</p>
 +
        <h1 id="main2">Progress</h1>
 +
        <h2>—January 2019</h2>
 +
        <p>The senior students publicized the iGEM and recruited candidates aspiring to participate iGEM representing ECUST-2019 iGEM team.</p>
 +
        <h2>—February 2019</h2>
 +
        <p>We had a brain storm within the iGEM club of ECUST.</p>
 +
        <h2>—April 2019</h2>
 +
        <p>We participated a meetup held at New York University Shanghai.</p>
 +
        <h2>—May 2019</h2>
 +
<p>We decided to focus on Paper Transformer as our project out of three others.</p>
 +
<p>We were invited to Jiangnan University in Wuxi, Jiangsu province to discuss our project. We communicated with three teams of Jiangnan University on human practices and gene circuit design.
 +
</p>
 +
        <h2>—June 2019</h2>
 +
        <p>We started to plan our wet lab arrangement and construction work.</p>
 +
        <h2>—July 2019</h2>
 +
        <p>Our experiment didn’t start until this month, but we quickly assigned the tasks into several team member. In detail, shows as follows:</p>
 +
        <ul class="circle">
 +
        <li><b>Cellulose degrading ability</b>
 +
        <p>This part was mostly undertaken by Hushijia, and  later assisted by Xianglan. The results could be seen at <a href="https://2019.igem.org/Team:ECUST_China/Results" target="_blank" style="text-decoration: underline;"> here<img style="height: 20px;" src="https://static.igem.org/mediawiki/2019/b/bd/T--ECUST_China--icon_cursor.png"></a>. </p>
 +
        <p>We firstly attempted to construct the cex and cenA on the pET-28b separately, since the dual inverter plasmid has not been constructed and the pET-28b is a reliable plasmid to express heterologous protein.</p>
 +
        <p>At the beginning, we got desired parts from kit by PCR very easily. And through Gibson-assembly method, we ligated the parts we purified. When we tested the ligation section by test primer and first generation sequencing, nonetheless,  there were not a single clone  comprise all the fragments we expected to be ligated.(More details please click the botton below)</p>
 +
        <div class="btn"><a href="https://static.igem.org/mediawiki/2019/8/8d/T--ECUST_China--experiments_protocols.pdf"> <b>Protocols</b></a></div> 
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        </li>
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        <li>
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        <!--here的链接框架已放 链接不明，更换即可 -->
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        <p><b>Cellulose producing ability</b></p>
 +
        <p>This part was firstly undertaken by Xianglan, and then taken over by Jinqianwei and  Miaozongjie. The results could be seen at<a href="https://2019.igem.org/Team:ECUST_China/Results" target="_blank" style="text-decoration: underline;"> here<img style="height: 20px;" src="https://static.igem.org/mediawiki/2019/b/bd/T--ECUST_China--icon_cursor.png"></a>.</p>
 +
          <p>We tried to purchase Gluconacetobacter xylinus from</p>
 +
        </li>
 +
        <li>
 +
        <p><b>Inversion-control ability</b></p>
 +
        <p>This part was mostly undertaken by Jinqianwei and  Miaozongjie. The results could be seen at <a href="https://2019.igem.org/Team:ECUST_China/Results" target="_blank" style="text-decoration: underline;">here<img style="height: 20px;" src="https://static.igem.org/mediawiki/2019/b/bd/T--ECUST_China--icon_cursor.png"></a>.</p>
 +
        </li>
 +
        </ul>
 +
        <h2>—August 2019</h2>
 +
        <ul class="circle">
 +
        <li><p><b>Cellulose degrading ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Cellulose producing ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Inversion-control ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
    </ul>
 +
      <h2>—September 2019</h2>
 +
        <ul class="circle">
 +
        <li><p><b>Cellulose degrading ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Cellulose producing ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Inversion-control ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
    </ul>
 +
          <h2>—October 2019</h2>
 +
        <ul class="circle">
 +
        <li><p><b>Cellulose degrading ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Cellulose producing ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
        <li><p><b>Inversion-control ability</b></p>
 +
              <p>that</p>
 +
        </li>
 +
    </ul> 
 +
      <p>More details about our notebook please click</p> 
 +
        <div class="btn"><a href="https://static.igem.org/mediawiki/2019/a/ac/T--ECUST_China--notebook.pdf"> <b>Notebook</b></a></div> 
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</div></div></div>
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  <br><br><br><br>
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<div id="contact">
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    <div class="cont-left">
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    <h4>ECUST_China</h4>
 +
<p style="text-align: right;">EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY</p>
 +
    <p style="text-align: right;">Shanghai, China</p>
 +
    <a href="https://www.ecust.edu.cn/"><img src="https://static.igem.org/mediawiki/2019/2/27/T--ECUST_China--ecust_school-w.png"> </a>
 +
    <a href="http://biotech.ecust.edu.cn/"><img src="https://static.igem.org/mediawiki/2019/3/33/T--ECUST_China--bioenger_logo-w.png"> </a> 
 +
   
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    </div>
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    <div class="cont-right">
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    <h4>GET IN TOUCH</h4>
 +
    <p>+86 021-64253306</p>
 +
    <p>ecust_igem_2019@163.com</p>
 +
    <a href="https://www.instagram.com/ecustigem2019/"><img src="https://static.igem.org/mediawiki/2019/4/4e/T--ECUST_China--contact_ins-w.png"></a>
 +
<a href="http://www.bilibili.com/video/av70010991?share_medium=android&share_source=more&bbid=XZ86E0483CEBD7A2D614212F77CE7C17C372B&ts=1571405282891"><img src="https://static.igem.org/mediawiki/2019/1/13/T--ECUST_China--contact_bilibili-w.png"></a>
 +
<a href="https://mp.weixin.qq.com/s/53fS8TxvqSTUhNPkjtXE_A"><img src="https://static.igem.org/mediawiki/2019/f/f4/T--ECUST_China--contact_wechat-w.png"></a>
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    </div>
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  </div>  
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  </div>
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<div id="gotop">
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  <img src="https://static.igem.org/mediawiki/2019/3/32/T--ECUST_China--back_top.png">
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  <script>
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      $(function(){
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var h3_height = $(".mininav-left").offset().top;
  
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<div class="column full_size">
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$(window).scroll(function(){
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var this_scrollTop = $(this).scrollTop();
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<h1>Notebook</h1>
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if(this_scrollTop>550 ){
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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$(".mininav-left").show(500);
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<h3>What should this page have?</h3>
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});
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<ul>
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$(function(){
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<li>Chronological notes of what your team is doing.</li>
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$("#gotop").click(function(){
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<li> Brief descriptions of daily important events.</li>
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$('body,html').animate({scrollTop:0},1000);
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<li>Pictures of your progress. </li>
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return false;
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<li>Mention who participated in what task.</li>
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</body>
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<div class="column third_size">
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p>You can see what others teams have done to organize their notes:</p>
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<ul>
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<li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</ul>
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</div>
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</div>
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Revision as of 17:18, 21 October 2019

Introduction

Paper Transformer is a superhero we made to change the paper world, and several of team members devoted most of their time and energy this season.

We assigned four iGEMers to achieve three abilities of our Paper Transformer. In detail, we assigned Hu Shijia for cellulose degrading module, Xiang Lan for cellulose producing module, Miao Zongjie and Jin Qianwei for inverter module.

Progress

—January 2019

The senior students publicized the iGEM and recruited candidates aspiring to participate iGEM representing ECUST-2019 iGEM team.

—February 2019

We had a brain storm within the iGEM club of ECUST.

—April 2019

We participated a meetup held at New York University Shanghai.

—May 2019

We decided to focus on Paper Transformer as our project out of three others.

We were invited to Jiangnan University in Wuxi, Jiangsu province to discuss our project. We communicated with three teams of Jiangnan University on human practices and gene circuit design.

—June 2019

We started to plan our wet lab arrangement and construction work.

—July 2019

Our experiment didn’t start until this month, but we quickly assigned the tasks into several team member. In detail, shows as follows:

  • Cellulose degrading ability

    This part was mostly undertaken by Hushijia, and later assisted by Xianglan. The results could be seen at here.

    We firstly attempted to construct the cex and cenA on the pET-28b separately, since the dual inverter plasmid has not been constructed and the pET-28b is a reliable plasmid to express heterologous protein.

    At the beginning, we got desired parts from kit by PCR very easily. And through Gibson-assembly method, we ligated the parts we purified. When we tested the ligation section by test primer and first generation sequencing, nonetheless, there were not a single clone comprise all the fragments we expected to be ligated.(More details please click the botton below)

    Protocols

  • Cellulose producing ability

    This part was firstly undertaken by Xianglan, and then taken over by Jinqianwei and Miaozongjie. The results could be seen at here.

    We tried to purchase Gluconacetobacter xylinus from

  • Inversion-control ability

    This part was mostly undertaken by Jinqianwei and Miaozongjie. The results could be seen at here.

—August 2019

  • Cellulose degrading ability

    that

  • Cellulose producing ability

    that

  • Inversion-control ability

    that

—September 2019

  • Cellulose degrading ability

    that

  • Cellulose producing ability

    that

  • Inversion-control ability

    that

—October 2019

  • Cellulose degrading ability

    that

  • Cellulose producing ability

    that

  • Inversion-control ability

    that

More details about our notebook please click

Notebook




ECUST_China

EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Shanghai, China

GET IN TOUCH

+86 021-64253306

ecust_igem_2019@163.com