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− | These constructs were systematically designed to experimentally test the expression and secretion of PETase. Additionally, we designed the constructs with different markers for detection or measurement of the enzyme, like the yellow fluorescent protein mVenus, HA-tags and His-tags. Construct <a href=”http://parts.igem.org/Part:BBa_K2984028”>BBa_K2984028 </a> was for example designed for enhanced secretion of PETase, marked with the YFP mVenus. Or construct <a href=”http://parts.igem.org/Part:BBa_K2984039”>BBa_K2984039</a>, designed for expression of the enzyme, marked with a HA-tag for detection, isolation and purification. For more constructs you can visit our <a href=”https://2019.igem.org/Team:Humboldt_Berlin/Composite_Part”>composite parts</a>page. | + | These constructs were systematically designed to experimentally test the expression and secretion of PETase. Additionally, we designed the constructs with different markers for detection or measurement of the enzyme, like the yellow fluorescent protein mVenus, HA-tags and His-tags. Construct <a href=”http://parts.igem.org/Part:BBa_K2984028”>BBa_K2984028 </a> was for example designed for enhanced secretion of PETase, marked with the YFP mVenus. Or construct <a href=”http://parts.igem.org/Part:BBa_K2984039”>BBa_K2984039</a>, designed for expression of the enzyme, marked with a HA-tag for detection, isolation and purification. For more constructs you can visit our <a href=”https://2019.igem.org/Team:Humboldt_Berlin/Composite_Part”>composite parts</a> page. |
| </p> | | </p> |
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