Difference between revisions of "Team:Humboldt Berlin/Parts"

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                                                         <td> L1-PsaD-GLE-PETase-YFP-SP20-RbcS2</td>
 
                                                         <td> L1-PsaD-GLE-PETase-YFP-SP20-RbcS2</td>
 
                                                         <td> <a href="http://parts.igem.org/Part:BBa_K2984029">BBa_K2984029 </a> </td>
 
                                                         <td> <a href="http://parts.igem.org/Part:BBa_K2984029">BBa_K2984029 </a> </td>
 +
                                                        <td>Coding</td>
 +
                                                        <td> Secretion of PETase and expression measurement via YFP fluorescence </td>
 +
                                     
 +
                                                    </tr>
 +
                                                    <tr>
 +
                                                        <td> L1-PsaD-ARS-PETase-YFP-RbcS2</td>
 +
                                                        <td> <a href="http://parts.igem.org/Part:BBa_K2984031">BBa_K2984031 </a> </td>
 
                                                         <td>Coding</td>
 
                                                         <td>Coding</td>
 
                                                         <td> Secretion of PETase and expression measurement via YFP fluorescence </td>
 
                                                         <td> Secretion of PETase and expression measurement via YFP fluorescence </td>

Revision as of 10:08, 18 October 2019

Building Blocks

Parts

Partbox

Part Name Registry Name Registry Type Description Fusion Sites Position in L1
L0 - Backbone BBa_K2984010 Plasmid L0 backbone (plasmid with RFP insert) - ▇
L0-AR BBa_K2984009 Regulatory L0 backbone with AR promoter A1-A3 ▇
L0-AR BBa_K2984025 Regulatory L0 backbone with AR promoter A1-B1 ▇
L0-PsaD BBa_K2984022 Regulatory L0 backbone with PsaD promoter A1-A3 ▇
L0-PsaD BBa_K2984008 Regulatory L0 backbone with PsaD promoter A1-B1 ▇
L0-ARS1 BBa_K2984000 Coding L0 backbone with 3'UTR Arylsulfatase1 secretion signal B2-B2 -
L0-GLE BBa_K2984001 Coding L0 backbone with 3'UTR gametolysin secretion signal B2-B2 -
L0-PETase BBa_K2984011 Coding L0 backbone with PETase CDS B3-B4 -
L0-Hyg BBa_K2984012 Coding L0 backbone with Hygromycin B resistance CDS B3-B4 -
L0-Paro BBa_K2984006 Coding L0 backbone with Paromomycine resistance CDS B3-B4 -
L0-ptxD BBa_K2984026 Coding L0 backbone with phosphite oxidoreductase CDS B3-B3 -
L0-ptxD BBa_K2984013 Coding L0 backbone with phosphite oxidoreductase CDS B3-B4 -
L0-YFP BBa_K2984017 Coding L0 backbone with yellow fluorescent protein CDS B3-B4 -
L0-YFP BBa_K2984024 Coding L0 backbone with yellow fluorescent protein CDS B4-B4 -
L0-YFP BBa_K2984020 Coding L0 backbone with yellow fluorescent protein CDS B5-B5 -
L0-6xHis tag BBa_K2984014 Tag L0 backbone with 6x histidine tag B5-B5 -
L0-3xHis tag BBa_K2984015 Tag L0 backbone with 3x histidine tag B5-B5 -
L0-SP20 BBa_K2984016 Coding L0 backbone with glycomodule for secretion enhancement B5-B5 -
L0-RbcS2 BBa_K2984018 Terminator L0 backbone with RuBisCo terminator B5-C1 -
Part Name Registry Name Registry Type Description
L1a BBa_K2984003 Plasmid backbone Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site
L1b BBa_K2984005 Plasmid backbone Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site
L1c BBa_K2984002 Plasmid backbone Level 1 backbone with ampicillin resistance and RFP flanked by GoldenGate restiction site



Part Name Registry Name Registry Type Description
L1-PsaD-Paro-RbcS2 BBa_K2984055 Coding Construct lending paromomycin resistance to C. reinhardtii
L1-PsaD-Hyg-RbcS2 BBa_K2984056 Coding Construct lending hygromycin resistance to C. reinhardtii
L1-PsaD-ARS-PETase-YFP-SP20-RbcS2 BBa_K2984028 Coding Secretion of PETase and expression measurement via YFP fluorescence
L1-PsaD-GLE-PETase-YFP-SP20-RbcS2 BBa_K2984029 Coding Secretion of PETase and expression measurement via YFP fluorescence
L1-PsaD-ARS-PETase-YFP-RbcS2 BBa_K2984031 Coding Secretion of PETase and expression measurement via YFP fluorescence