</li>

</li>

<li>05

<li>05

</li>

</li>

</ul>

</ul>

</div>

</div>

<div class="mian-text-box">

<div class="mian-text-box">

<p>As the idea of sustainable development prevails, <b>paper recycling

<p>As the idea of sustainable development prevails, <b>paper recycling

  campaigns</b>  have been gaining momentum. Numerous paper mills have put waste paper into reuse, attempting to cut the cost. The paper fiber, nevertheless, cannot be recycled infinitely because of <b>keratinization</b> during pulp-beating, and those broken short fibers are <b>discarded</b> in each round of recycling process. Our Paper-transformer is capable of transforming these <b>TRASH</b> into <b>TREASURE</b>!

  campaigns</b>  have been gaining momentum. Numerous paper mills have put waste paper into reuse, attempting to cut the cost. The paper fiber, nevertheless, cannot be recycled infinitely because of <b>keratinization</b> during pulp-beating, and those broken short fibers are <b>discarded</b> in each round of recycling process. Our Paper-transformer is capable of transforming these <b>TRASH</b> into <b>TREASURE</b>!

<img class="img-com" src="https://static.igem.org/mediawiki/2019/1/14/T--ECUST_China--demonstrate_bio-process.svg">

<img class="img-com" src="https://static.igem.org/mediawiki/2019/1/14/T--ECUST_China--demonstrate_bio-process.svg">

</div>

</div>

<p>We have equipped the our chasis organism—E.coli—with three types of cellulase—<b>β-glycosidase, endoglucanase, exoglucanase</b>,

<p>We have equipped the our chasis organism—E.coli—with three types of cellulase—<b>β-glycosidase, endoglucanase, exoglucanase</b>,

  encoded by gene cep94A, cenA, cex respectively, which work as a whole to facilitate the host bacterium to degrade cellulose into glucose. Yet we didn’t fantasize the glucose could accumulate gradually as a stable intermediate without being utilized by the host cell, given its ready availability as energy source beyond others. So we decided to stop at cellobiose, a disaccharide produced from cellulose by exoglucanase and endoglucanase combine and catalyzed into glucose by β-glycosidase.

  encoded by gene cep94A, cenA, cex respectively, which work as a whole to facilitate the host bacterium to degrade cellulose into glucose. Yet we didn’t fantasize the glucose could accumulate gradually as a stable intermediate without being utilized by the host cell, given its ready availability as energy source beyond others. So we decided to stop at cellobiose, a disaccharide produced from cellulose by exoglucanase and endoglucanase combine and catalyzed into glucose by β-glycosidase.

</p>

</p>

</div>

</div>

<p>We have also imparted the our chasis organism—E.coli—with cellulose synthase and its accessory proteins, encoded by gene acsAB, acsC and acsD respectively. </p>

<p>We have also imparted the our chasis organism—E.coli—with cellulose synthase and its accessory proteins, encoded by gene acsAB, acsC and acsD respectively. </p>

</div>

</div>

<p>Since cellulase and cellulose synthase function as a pair of

<p>Since cellulase and cellulose synthase function as a pair of

  antagonists, consuming each other’s product and build their own, it is pivotal to prevent this futile and relentless competition from happening. Thus, we designed an inverter, a duel plasmid system which is compatible in the DH5α host cell, to separate the degradation of cellulose from accumulation of Bacterial cellulose, and to

  antagonists, consuming each other’s product and build their own, it is pivotal to prevent this futile and relentless competition from happening. Thus, we designed an inverter, a duel plasmid system which is compatible in the DH5α host cell, to separate the degradation of cellulose from accumulation of Bacterial cellulose, and to

         <img class="img-com" src="https://static.igem.org/mediawiki/2019/3/31/T--ECUST_China--diplasmid_circuit.svg">

         <img class="img-com" src="https://static.igem.org/mediawiki/2019/3/31/T--ECUST_China--diplasmid_circuit.svg">

</div>

</div>

</div>

</div>

</div>

</div>

<div id="gotop">

<div id="gotop">

   <img src="https://static.igem.org/mediawiki/2019/3/32/T--ECUST_China--back_top.png">

   <img src="https://static.igem.org/mediawiki/2019/3/32/T--ECUST_China--back_top.png">