Difference between revisions of "Team:Humboldt Berlin/Notebook"
| Line 116: | Line 116: | ||
- Research on PETase degradation <br> | - Research on PETase degradation <br> | ||
- Planning construction design for Golden Gate cloning using 10 different fusion sites according to Patron <br> | - Planning construction design for Golden Gate cloning using 10 different fusion sites according to Patron <br> | ||
| − | - Designing Primers for C. reinhardtii specified promotor PsaD containing BpiI recognition site with MoClo fusion sites and fusion sites compatible for L0 backbone. Primers containing mutation to delete BpiI site (Primer 5,6,11-13)." | + | - Designing Primers for <i>C. reinhardtii</i> specified promotor PsaD containing BpiI recognition site with MoClo fusion sites and fusion sites compatible for L0 backbone. Primers containing mutation to delete BpiI site (Primer 5,6,11-13)." |
data-date="10/08 - 10/14" | data-date="10/08 - 10/14" | ||
data-category="synthesis"> | data-category="synthesis"> | ||
| Line 122: | Line 122: | ||
</div> | </div> | ||
<div class="timeline-dot" | <div class="timeline-dot" | ||
| − | data-text="- Research on C. reinhardtii cultivation <br> | + | data-text="- Research on <i>C. reinhardtii</i> cultivation <br> |
<h2>YFP</h2> | <h2>YFP</h2> | ||
- Designing Primers for YFP with B5-B5 fusion sites taken from AG Hegemann plasmid p135 (Primer 7+8)" | - Designing Primers for YFP with B5-B5 fusion sites taken from AG Hegemann plasmid p135 (Primer 7+8)" | ||
| Line 386: | Line 386: | ||
data-text="<div class='two-columns'><div> | data-text="<div class='two-columns'><div> | ||
<h2>Paromomycin</h2> | <h2>Paromomycin</h2> | ||
| − | - Preparation of 3 overnight cultures of | + | - Preparation of 3 overnight cultures of clones 1, 2, 3 (colony PCR 22.02.) <br> |
| − | + | - Plasmid prep the next day<br> | |
| − | + | - Sending plasmids for sequencing <br> | |
- Corrected sequencing results of L0-Paro B3-B4 | - Corrected sequencing results of L0-Paro B3-B4 | ||
<h2>Hygromycin</h2> | <h2>Hygromycin</h2> | ||
| Line 398: | Line 398: | ||
<div> | <div> | ||
<h2>L1a,b,c - RFP</h2> | <h2>L1a,b,c - RFP</h2> | ||
| − | - mistake: it was noticed that primer to amplify 3 different RFP versions were designed wrong → buffer bases were missing, so that the restriction enzymes did not | + | - mistake: it was noticed that primer to amplify 3 different RFP versions were designed wrong → buffer bases were missing, so that the restriction enzymes did not attach to the DNA <br> |
- designing and ordering new primers containing buffer bases (primer 84-89) | - designing and ordering new primers containing buffer bases (primer 84-89) | ||
</div></div>" | </div></div>" | ||
Revision as of 10:56, 16 October 2019
Notebook
Select category
October
2018
2018
November
December
January
February
March
May
January 2019
May
June
July
October
November 2018
December
January