Difference between revisions of "Team:Humboldt Berlin/Contribution"

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<h3 class="headline3">Characterizing Chromo-Proteins</h3>                         
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<h3 class="headline3">Characterizing Chromoproteins</h3>                         
 
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We encountered problems when measuring the the YFP-signal in our transformed C. rheinhardtii YFP strains, even though we were able to detect YFP when examining the cells with the fluorescence microscope. To get a better understanding of the protocols followed to measure absorbance and fluorescence spectra with a plate reader, we conducted some exemplary measurements with fluorescent and non-fluorescent chromoproteins expressed in E. coli.  
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We encountered problems when measuring the the YFP-signal in our transformed <i>C. rheinhardtii</i> YFP strains, even though we were able to detect YFP when examining the cells with the fluorescence microscope. To get a better understanding of the protocols followed to measure absorbance and fluorescence spectra with a plate reader, we conducted some exemplary measurements with fluorescent and non-fluorescent chromoproteins expressed in <i>E. coli</i>.  
 
We tested three different chromoproteins from the iGEM Registry of Standard Biological Parts in terms of their absorption spectra (as well as the fluorescence spectrum in case of amajLime): <br/>
 
We tested three different chromoproteins from the iGEM Registry of Standard Biological Parts in terms of their absorption spectra (as well as the fluorescence spectrum in case of amajLime): <br/>
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<li>amajLime from the coral Anemonia majato <a href="http://parts.igem.org/Part:BBa_K1033914">BBa_K1033914 </a></li>
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<li>amajLime from the coral <i>Anemonia majato</i> <a href="http://parts.igem.org/Part:BBa_K1033914">BBa_K1033914 </a></li>
 
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Revision as of 15:52, 15 October 2019

Colorporteins

Characterization

It's colorfull

Characterizing Chromoproteins

We encountered problems when measuring the the YFP-signal in our transformed C. rheinhardtii YFP strains, even though we were able to detect YFP when examining the cells with the fluorescence microscope. To get a better understanding of the protocols followed to measure absorbance and fluorescence spectra with a plate reader, we conducted some exemplary measurements with fluorescent and non-fluorescent chromoproteins expressed in E. coli. We tested three different chromoproteins from the iGEM Registry of Standard Biological Parts in terms of their absorption spectra (as well as the fluorescence spectrum in case of amajLime):

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