To be able to work with C. reinhardtii, you will need medium. This medium can be in either liquid or solid form and is essential for any experiment regarding C. reinhardtii. The media we used for C. reinhardtii is TAP medium.

Media	0,5 L	1,8L
40 x TAP/TA/TAPi	12,5 ml	20 ml
40 x Beijerink Salts	12,5 ml	20 ml
Trace Elements (revised)	3,5 ml	5,6 ml
H2O	471,5 ml	754,4 ml
For Plates: + Agar (1,8 %)	7,2 g	14,4 g

Tap Medium should be adjusted to 7,0 - 7,2 pH and autoclaved.

40 x TAP 1L	autoclave, store at 4°C
Tris (121,14 g/mol)	96,8 g (0,8 M)
1 M (K) PO4	40 ml (40 mM)

adjust pH 7.0-7.2 with conc. AcOH (~44ml)

40 x TA 1L	autoclave, store at 4°C
Tris (121,14 g/mol)	96,8 g (0,8 M)

adjust pH 7.0-7.2 with conc. AcOH (~44ml)

40 x TAPi 1L	autoclave, store at 4°C
Tris (121.14 g/mol)	96,8 g (0,8 M)
1 M K2HPO3 120.08 g/mol	40ml (40mM)

adjust pH 7.0-7.2 with conc. AcOH (~44ml)

1 M (K)PO4	MW(g/mol)	conc.	420 ml
K2HPO4 ∙3 H2O	228,22	1 M; 228 g/l	≈0,6 M; 136 g/l
K2HPO4	136,09	1 M; 136 g/l	≈ 0,4 M; 55,08 g/l

250 ml 1 M K₂HPO₄ + ca. 170 ml KH₂PO₄ , adjust to pH 7, autoclave, store at 4°C

1 M (K)PO3	MW(g/mol)	conc.	1 l
K2HPO3	120.086	1 M; 120.086 g/l	≈1 M; 120.086 g/l

K₂HPO₃ was obtained by Haihang co. ltd. (13977-65-6) Autoclave, store at 4°C

40 x Beijerinck salts 1 L	autoclave, store at 4°C
NH 4Cl	16 g
CaCl2 ∙2H 2O	2 g
MgSO 4 ∙7H 2O	4 g

Trace Elements (revised)

To make the trace elements following publication was used: Kropat, J., Hong-Hermesdorf, A., Casero, D., Ent, P., Castruita, M., Pellegrini, M., …
Malasarn, D. (2011). A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii.
The Plant Journal, 66(5), 770–780. http://doi.org/10.1111/j.1365-313X.2011.04537.x

Additive (optional)	Working Concentration
Arginin	100 µg/ml
Paromomycin	10-12 µg/ml
Hygromycin	10 µg/ml
Ampicillin	100-500 µg/ml

TODO

TODO