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| <p>We attended Lincoln Southeast High School. We gave a presentation on our project and informed the students of an advanced chemistry class about synthetic biology and its applications in the iGEM competition. The students seemed increasingly engaged and asked a lot of insightful questions. In this way we were able to connect with the potential beneficiaries of our project and get input on how it might affect their lives in the future. We were also able to foster some level of interest in genetic engineering and synthetic biology, allowing for a new generation of iGEM teams.</p> | | <p>We attended Lincoln Southeast High School. We gave a presentation on our project and informed the students of an advanced chemistry class about synthetic biology and its applications in the iGEM competition. The students seemed increasingly engaged and asked a lot of insightful questions. In this way we were able to connect with the potential beneficiaries of our project and get input on how it might affect their lives in the future. We were also able to foster some level of interest in genetic engineering and synthetic biology, allowing for a new generation of iGEM teams.</p> |
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| <p>Dr. Somerville is an associate professor at the School of Veterinary Medicine and Biomedical Sciences at the University of Nebraska—Lincoln. He specializes in bacterial physiology and pathogenesis. We visited him near the beginning of the project to learn more about Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA). He provided information about the threat of MRSA to the global community and the potential effectiveness of our project. Dr. Somerville indicated that MRSA is a growing threat in places where skin-to-skin contact is prevalent, such as hospitals and sports complexes. He explained that the spread of MRSA is on the rise and will be a serious threat to public health by 2050. He addressed our project as a potentially feasible idea and offered suggestions for improvement. Rather than our original goals of in vivo usage, Dr. Somerville believed in vitro applications would be more appropriate on the basis of safety and practicality. He also advised that our Gram-negative chassis, <i> E. coli</i>, may struggle to detect AIP. Since we were unable to work with a Gram-positive chassis, we developed a protocol to permeabilize the outer membrane of our cells. This was intended to enhance the ability of <i>E. coli</i> to detect AIP. More information about Dr. Somerville can be found <a href="https://vbms.unl.edu/greg-somerville">here.</a><p> | | <p>Dr. Somerville is an associate professor at the School of Veterinary Medicine and Biomedical Sciences at the University of Nebraska—Lincoln. He specializes in bacterial physiology and pathogenesis. We visited him near the beginning of the project to learn more about Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA). He provided information about the threat of MRSA to the global community and the potential effectiveness of our project. Dr. Somerville indicated that MRSA is a growing threat in places where skin-to-skin contact is prevalent, such as hospitals and sports complexes. He explained that the spread of MRSA is on the rise and will be a serious threat to public health by 2050. He addressed our project as a potentially feasible idea and offered suggestions for improvement. Rather than our original goals of in vivo usage, Dr. Somerville believed in vitro applications would be more appropriate on the basis of safety and practicality. He also advised that our Gram-negative chassis, <i> E. coli</i>, may struggle to detect AIP. Since we were unable to work with a Gram-positive chassis, we developed a protocol to permeabilize the outer membrane of our cells. This was intended to enhance the ability of <i>E. coli</i> to detect AIP. More information about Dr. Somerville can be found <a href="https://vbms.unl.edu/greg-somerville">here.</a><p> |
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