Basic Parts
| Name | Number | Type | Description | Designer | Length |
|---|---|---|---|---|---|
| SarA | BBa_K3191100 | Regulatory | A transcriptional activator in the agr system in S. aureus. This binds and activates P2 and P3 promoters in the presence of AgrA. | Logan Hauder | 378 |
| AgrC | BBa_K3191110 | Coding | AgrC interacts with autoinducing peptides, triggering phosphorylation of AgrA in the agr system of S. aureus. This part is a modification of the agrC subunit in BBa_I746100 that includes an additional 16 amino acids at the N-terminus of the peptide. | Logan Hauder | 1293 |
| AgrA | BBa_K3191111 | Coding | AgrA activates P2 and P3 promoters in the agr system of S. aureus when phosphorylated by AgrC. This part is a modification of the agrA subunit in BBa_I746100 that includes an additional 30 amino acids at the N-terminus of the peptide. | Logan Hauder | 717 |
| YbaQ Degron | BBa_K3191200 | Tag | This part includes the YbaQ degron sequence with two stop codons added to the C-terminal end. | Alexander Meyer | 36 |
| GakA | BBa_K3191310 | Coding | Contains the sequence for gakA, the first of three subunits in the bacteriocin garvicin KS. | Alexander Meyer | 108 |
| GakB | BBa_K3191311 | Coding | Contains the sequence for gakB, the second of three subunits in the bacteriocin garvicin KS. | Alexander Meyer | 108 |
| GakC | BBa_K3191312 | Coding | Contains the sequence for gakC, the third of three subunits in the bacteriocin garvicin KS. | Alexander Meyer | 102 |
| Novel Secretion Peptide NSP4 | BBa_K3191320 | Coding | An N-terminal secretion tag for extracellular secretion of peptides in E. coli through the Sec-dependent pathway. | Logan Hauder | 60 |
| P2 Promoter | BBa_K3191400 | Regulatory | The P2 promoter is activated by AgrA in the agr system of S. aureus, transcribing genes downstream of the promoter in the presence of autoinducing peptides. This part is a modification of BBa_I746104, reintroducing about 90 missing bases in the intergenic sequence. | Alexander Meyer | 151 |
Composite Parts
| Name | Number | Type | Description | Designer | Length |
|---|---|---|---|---|---|
| pBAD + AgrC + AgrA + GFP with Multiple Cloning Site | BBa_K3191101 | Composite | This part contains gakA, gakB, and gakC under the influence of a pBAD promoter. This encourages the production of garvicin KS in the presence of arabinose. | Alexander Meyer | 3372 |
| pBAD + AgrC + AgrA + SarA + GFP | BBa_K3191102 | Composite | Includes the agrC and agrA genes from the agr system in S. aureus under a pBAD promoter. Downstream, a gene for GFP is under the influence of a P2 promoter. This is designed so AIP detection triggers production of GFP. This part includes SarA immediately downstream of AgrA to improve P2 transcription. | Logan Hauder | 3776 |
| SarA | BBa_K3191103 | Composite | This part includes a ribosome binding site immediately upstream of sarA for cloning into the BBa_K3191101 multiple cloning site. | Logan Hauder | 396 |
| pBAD + CheZ + YbaQ | BBa_K3191201 | Composite | A YbaQ degron sequence is attached to cheZ to prevent overexpression, improving motility. These genes are placed downstream of a pBAD promoter, encouraging chemotaxis in the presence of arabinose. | Alexander Meyer | 974 |
| pBAD + GarKS | BBa_K3191301 | Composite | This part includes the YbaQ degron sequence with two stop codons added to the C-terminal end. | Alexander Meyer | 666 |
| pBAD + GarKS + NSP4 | BBa_K3191302 | Composite | This part contains gakA, gakB, and gakC under the influence of a pBAD promoter, encouraging the production of garvicin KS in the presence of arabinose. This part also includes a short secretion peptide attached to each subunit of garvicin KS to help with extracellular secretion. | Alexander Meyer | 846 |
