![OPEN PBR LOGO](https://static.igem.org/mediawiki/2019/4/42/T--Humboldt_Berlin--openPBR.png)
Introduction
Producing reliable cultivation data can be fairly difficult, time consuming and pricey.
We decided to adress this problem by creating OPEN PBR, a modular cultivation setup for photosynthetic organisms with up to 9 separate cultivation vessels.
While gathering knowledge with our efforts in Human Practices, we quickly recognized the need for a platform that enables the user to vary many parameters during cultivation of microalgae while still being affordable and capable of generating reproducible data.
The OPEN PBR fulfills these demands by providing a modular setup for cultivation of algae in turbido- or chemostat mode. With all components defined an openly available, it remains highly modular while using standardized vessels to provide reproducibility.
By using an existing model of general equations describing a chemostat with light as limiting substrate, we were able to pin down necessary functions and devices for performing them. For easy assembly of the device, files for lasercutting, a list of components and where to order them and an assembly guideline are provided below.
![Our Open PBR](https://static.igem.org/mediawiki/2019/3/39/T--Humboldt_Berlin--beschriftet_open_pbr.png)
Chemostat equation
Light Gradient
OD Measurement
Designing the bioreactor
![Bringing Chlamy to iGEM](https://static.igem.org/mediawiki/2019/b/be/T--Humboldt_Berlin--microplastic_icon_ablauf.png)
Sensors
Illumination
Gas mixing and pumps
Cultivation vessel
Casing
Overview of single components
In this chapter, we give an overview for all functions and components of the system. The aim is to serve as an introduction for assembling the system with the instructions provided in the last chapter.
Github Project
Tools
PDF Instructions
Assembly Instructions
This chapter provides you with everything you need to rebuild our setup on your own - including software, a list of used parts and assembly instructions.
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