0 Introduction
Producing reliable cultivation data can be fairly difficult, time consuming and pricey.
We decided to adress this problem by creating OPEN PBR, a modular cultivation setup for photosynthetic organisms with up to 9 separate cultivation vessels.
While gathering knowledge with our efforts in Human Practices, we quickly recognized the need for a platform that enables the user to vary many parameters during cultivation of microalgae while still being affordable and capable of generating reproducible data.
The OPEN PBR fulfills these demands by providing a modular setup for cultivation of algae in turbido- or chemostat mode. With all components defined an openly available, it remains highly modular while using standardized vessels to provide reproducibility.
By using an existing model of general equations describing a chemostat with light as limiting substrate, we were able to pin down necessary functions and devices for performing them. For easy assembly of the device, files for lasercutting, a list of components and where to order them and an assembly guideline are provided.
1 Design of the open PBR
2 Overview of single components
3 Assembly instructions
1.3.2 Testing secretion signals
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sensors
illumination
gas mixing
pumps
cultivation vessel
casing
2 Overview of single components
2.1 Modeling PET degradation by C. reinhardtii using an optimized PETase
3 Assembly instructions
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