Difference between revisions of "Team:Humboldt Berlin/Notebook"

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                         May
 
                         May
 
                     </div>
 
                     </div>
 +
<div class="timeline-month"
 +
                        data-text="<h2>YFP</h2>
 +
                        - PCR of YFP B4-B4; size: 749 bp <br>
 +
                        - Ligation YFP B4-B4 with L0-RFP"
 +
                        data-image="https://static.igem.org/mediawiki/2019/f/f7/T--Humboldt_Berlin--notebook_s_0527.png"
 +
                        data-category="synthesis"
 +
                        data-date="05/27 - 06/02">
 +
                        June
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>YFP</h2>
 +
                        - Transformation L0-YFP B4-B4 into <i>E. coli </i><br>
 +
                        - Colony PCR YFP-B4-B4 in <i>E. coli</i>"
 +
                        data-category="synthesis"
 +
                        data-date="06/03 - 06/09">
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>YFP</h2>
 +
                        - sequencing result of YFP B4-B4 clone 2 was positive"
 +
                        data-category="synthesis"
 +
                        data-date="06/17 - 06/23">
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="<h2>B2 Linker</h2>
 +
                        - creating new primers"
 +
                        data-category="synthesis"
 +
                        data-date="07/01 - 07/07">
 +
                        July
 +
                    </div>
 +
                    <div class="timeline-dot"
 +
                        data-text="<h2>B2 Linker</h2>
 +
                        - Oligo Annealing B2 Linker<br>
 +
                        - succesfull"
 +
                        data-category="synthesis"
 +
                        data-image="https://static.igem.org/mediawiki/2019/e/e8/T--Humboldt_Berlin--notebook_s_0708.png"
 +
                        data-date="06/17 - 06/23">
 +
                    </div>
 +
 +
  
 
                     <!-- human practice -->
 
                     <!-- human practice -->
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                         data-category="chlamy">
 
                         data-category="chlamy">
 
                         January
 
                         January
 +
                    </div>
 +
<div class="timeline-month"
 +
                        data-text="- First Toxicity-tests in a multicultivator, which measures algal growth under different conditions: Inhibition of C. reinhardtii by ethylene glycol at a concentration of 6 % was confirmed<br>
 +
                        - Cultivation of strain CC-125 in high salt medium shows inhibited growth <br><br>
 +
                        - participants: Paul Herrmann, Andrej Andjelic"
 +
                        data-date="May 2019"
 +
                        data-category="chlamy">
 +
                        May
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- started the transformations of L1-constructs into Chlamy<br>
 +
                        - checked the functionality of our paromomycin and hygromycin selection cassettes<br>
 +
                        - Cultivation tests of strain SAG 11-32b in high salt medium with added PET in concentrations of 10-800 mg/L. Wastewater contains 250 mg/L PET before treatment. <br><br>
 +
                        - participants: Simon Kelterborn, Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann"
 +
                        data-date="June 2019"
 +
                        data-image="https://static.igem.org/mediawiki/2019/4/40/T--Humboldt_Berlin--HSM_PET.jpeg"
 +
                        data-category="chlamy">
 +
                        June
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- First Chlamy transformations are discarded, since they were contaminated<br>
 +
                        - Transformation of further L1-constructs<br>
 +
                        - constructs containing YFP: colony-PCR showed integration of the plasmid into Chlamy’s genome, but no fluorescence could be measured by the plate reader <br>
 +
                        - PETase-YFP-containing constructs displayed fluorescence visible under the confocal microscopy<br><br>
 +
                        - participants: Darius Rauch, Dimitri Schumacher, Elena Rätsch, Juliana Rojas Pión, Paul Herrmann"
 +
                        data-date="July 2019"
 +
                        data-category="chlamy">
 +
                        July
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- Started with the construction of our own bioreactor. Conceptualization of the functionalities and necessary features had before been experimentally determined during our multicultivation tests<br>"
 +
                        data-date="August 2019"
 +
                        data-category="chlamy">
 +
                        August
 +
                    </div>
 +
                    <div class="timeline-month"
 +
                        data-text="- Wiring and programming the sensors and LEDs on the DIY-bioreactor<br>
 +
                        - laser-cutting of the shell and cultivation compartment of our bioreactor"
 +
                        data-date="September 2019"
 +
                        data-category="chlamy">
 +
                        September
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>

Revision as of 11:55, 19 October 2019

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Synthesis: Parts and Vectors
human practice
c. reinhardtii cultivation and transformations
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