Difference between revisions of "Team:Humboldt Berlin/Experiments"

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                                     To be able to work with C. reinhardtii, you will need medium.
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                                     To be able to work with <i>C. reinhardtii</i>, you will need medium.
 
                                     This medium can be in either liquid or solid form and is essential
 
                                     This medium can be in either liquid or solid form and is essential
 
                                     for any experiment regarding C. reinhardtii. The media we used for
 
                                     for any experiment regarding C. reinhardtii. The media we used for
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                                     C. reinhardtii is TAP medium.
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                                     <i>C. reinhardtii</i> is TAP medium.
 
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                     <h3 class="pink">E. coli Protocols</h3>
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                     <h3 class="pink"><i>E. coli</i> Protocols</h3>
  
 
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Revision as of 22:19, 1 October 2019

Experiments

Experiments

Protocolls

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Chlamydomonas Protocol

Media & Plates

To be able to work with C. reinhardtii, you will need medium. This medium can be in either liquid or solid form and is essential for any experiment regarding C. reinhardtii. The media we used for C. reinhardtii is TAP medium.

Media 0,5 L 1,8L
40 x TAP/TA/TAPi 12,5 ml 20 ml
40 x Beijerink Salts 12,5 ml 20 ml
Trace Elements (revised) 3,5 ml 5,6 ml
H2O 471,5 ml 754,4 ml
For Plates: + Agar (1,8 %) 7,2 g 14,4 g

Tap Medium should be adjusted to 7,0 - 7,2 pH and autoclaved.

Cultivation

TODO

Transformation

TODO

Colony Screening PCR

TODO

E. coli Protocols

Cultivation

Media & Plates

TODO

Overnight Cultures

TODO

Transformation

TODO

DNA-Isolation

TODO

Synthesis Protocols

Sequence & Primer Design

TODO

PCR

TODO

Digestion

TODO

Ligation

TODO

General

Gel Electrophoresis

TODO

Protein Expression: SDS-page

TODO

Workflow

Plasmid assembly

TODO