Difference between revisions of "Team:UNebraska-Lincoln/Collaborations"

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{{UNebraska-Lincoln}}
 
{{UNebraska-Lincoln}}
 
<html>
 
<html>
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    <head>
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            <meta charset="UTF-8">
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          <!-- Styles -->
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<div class="column full_size judges-will-not-evaluate">
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.body-content{
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<h3>★  ALERT! </h3>
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position: relative;
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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width: 100%;
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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background-color: white;
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<div class="column full_size">
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<h1>Collaborations</h1>
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  }
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  .dropdown li{
  
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<p>
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    font-size: 13pt;
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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    line-height: 2.0
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</p>
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  }
  
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<h3>Silver Medal Criterion #2</h3>
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  .body-background .background-text{
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<p>
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        padding-top: 140px;
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Document your collaboration(s) clearly on this page to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2019.igem.org/Judging/Medals">2019 Medals Page</a> for more information.  
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        text-align: center;
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</p>
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</div>
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<div class="column two_thirds_size">
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  }
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}
  
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<h4> Which other teams can we work with? </h4>
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figure img{
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<p>
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  display:block; /*remove inline-block spaces*/ 
−
You can work with any other team in the competition, including standard, software, open, and high school track teams. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
+
  margin:0 -18.885%;
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  width:120.777%;
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  opacity: 0.8;
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  filter: saturate(170%);
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}
  
−
<p>
 
−
In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
 
−
</p>
 
  
−
</div>
+
/**********************/
 +
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/**********************/
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padding-bottom: 0.5rem;
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<div class="column third_size">
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.footer .list-unstyled .fas {
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<p>
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color: #00bfd8;
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Here are some suggestions for projects you could work on with other teams:
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−
</p>
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line-height: 1.375rem;
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}
  
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<ul>
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.footer .list-unstyled .media-body {
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<li> Improve the function of another team's BioBrick Part or Device</li>
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margin-left: 0.625rem;
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<li> Characterize another team's part </li>
+
}
−
<li> Debug a construct </li>
+
−
<li> Model or simulate another team's system </li>
+
−
<li> Test another team's software</li>
+
−
<li> Help build and test another team's hardware project</li>
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−
<li> Mentor a high-school team</li>
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−
</ul>
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</div>
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        <div class="container">
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                <div class="body-background">
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                        <div class="background-text">
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                                <h1 style="color:white;
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                                font-weight: bold;
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                                padding-top: 50px;
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                                font-size: 40pt;">Collaborations</h1>
 +
                        </div>
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                </div>
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                <div class="body-content" data-spy="scroll" data-target=".navbar" data-offset="50">
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                                        <div class="container-fluid">
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                                          <div>
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                                            <div class="collapse navbar-collapse" id="myNavbar">
 +
                                              <ul class="nav nav-pills flex-column">
 +
                                                <li><a href="#section1">Northwestern University </a></li>
 +
                                                <li><a href="#section2">Purdue</a></li>
 +
                                                <li><a href="#section3">Jillin China</a></li>
 +
                                              </ul>
 +
                                            </div>
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                                          </div>
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                                    </div>
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                                    <div class="col-xs-8">
 +
                                      <div class="row">
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                                        <div id="section1" class="col-xs-12">
 +
                                          <h1>Northwestern University </h1>
 +
                                          <br/>
 +
                                          <br/>
 +
                                          <figure>
 +
                                           
 +
                                                <img src="https://static.igem.org/mediawiki/2019/e/ee/T--UNebraska-Lincoln--NU3.jpg">
 +
                                           
 +
                                            </figure>
 +
                                            <br/>
 +
                                            <br/>
 +
                                          <p>
 +
                                                Northwestern is working on a project to visualize UV damage on the skin. This works using the natural SOS mechanism that responds to damage by stopping the cell cycle and activating DNA repair mechanisms. Northwestern is working towards marking this response using a biological circuit hosted by E-coli, they are using GFP as an indicator. We partnered with Northwestern to work towards getting a biobrick (BBa_J04454) they planned on using as part of an internal reference control system to function properly. They were having trouble transforming the biobrick into E-coli using the DH5ɑ strain, so we reattempted the transformation using our own strain of E-coli, genehog. The transformation was successful.
 +
                                                </p>
 +
                                                <p>
 +
                                                To ensure the success of the part and the functionality of the mCherry gene that was needed by Northwestern, we conducted a fluorescence characterization tracking expression over a period of 4 hours. This yielded positive results. A two-tailed t-test of 0.05 significance between Hour 0 and Hour 3 produced a <span style="text-decoration:underline;">p-value of 0.000135</span>. This indicates there was significant expression of the mCherry gene over the course of the experiment. To learn more about our collaboration with Northwestern University and the details of their project, click <a href="https://2019.igem.org/Team:Northwestern/Collaborations" target="_blank">here.</a>
 +
                                                </p>
 +
                                          <h2 style="text-decoration:underline;">Results</h2>
 +
                                          <br/>
 +
                                            <br/> 
 +
                                          <img style="padding-left: 30%; filter: saturate(180%);" src="https://static.igem.org/mediawiki/2019/3/34/T--UNebraska-Lincoln--NU1.jpg">   
 +
                                          <br/> 
 +
                                          <br/> 
 +
                                          <br/> 
 +
                                          <img style="padding-left: 20%; filter: saturate(180%);" src="https://static.igem.org/mediawiki/2019/2/21/T--UNebraska-Lincoln--NU2.jpg">   
 +
                                       
 +
                                        </div>
 +
                                      </div>
 +
                                      <br/> 
 +
                                          <br/> 
 +
                                          <br/>
 +
                                          <br/> 
 +
                                          <br/> 
 +
                                          <br/>
 +
                                      <div class="row">
 +
                                            <div id="section2" class="col-xs-12">
 +
                                                   
 +
                                              <h1>Purdue </h1>
 +
                                              <br/>
 +
                                              <br/>
 +
                                              <figure>
 +
                                                 
 +
                                                        <img style="  display:block; /*remove inline-block spaces*/ 
 +
                                                    height:500px;
 +
                                                    width:100%;
 +
                                                    margin:unset;
 +
                                                    transform: scale(1.2);
 +
                                                    opacity: 0.8;
 +
                                                    filter: saturate(120%);" src="https://static.igem.org/mediawiki/2019/6/6f/T--UNebraska-Lincoln--purdue.jpeg">
 +
                                               
 +
                                                </figure>
 +
                                                <br/>
 +
                                                <br/>
 +
                                             
 +
<p>
 +
        The Purdue University iGEM team is working on combating the <em>M. oryzae </em>fungal infection which infects rice plants and results in a significantly decreased crop yield. In order to fight <em>M. oryzae’</em>s ability to bypass the rice plants’ immune response, Purdue is attempting to instigate a preemptive response. To learn more about the project click <a href="https://2019.igem.org/Team:Purdue/Description" target="_blank">here.</a>.
 +
        </p>
 +
        <p>
 +
        We conference called with Purdue and discussed troubleshooting our respective projects, they offered us advice on protocol alterations that might help with our characterization of BBa_K584000. In turn, we offered advice on trouble they were having with the formatting of their website.
 +
        </p>
 +
                                               
 +
                                            </div>
 +
                                          </div>
 +
                                          <br/> 
 +
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                                          <br/> 
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 +
                                     
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                                          <div class="row">
 +
                                                <div id="section3" class="col-xs-12">
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                                                  <h1>Jillin China </h1>
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<p>
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        The Jillin iGEM team set out to develop a system to sense and then subsequently inhibit <em>Candida albicans</em> infection, what is more commonly referred to as a yeast infection. We conferenced with Jillin towards the beginning of both our projects and discussed potential routes of development we could both take.
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                            <h4>Address</h4>
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                            <p>University of Nebraska-Lincoln iGEM Team
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                            <br />634 Hamilton Hall
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                            <br />Lincoln, NE 68588-0304</p>
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Latest revision as of 07:16, 9 December 2019

Collaborations

Northwestern University





Northwestern is working on a project to visualize UV damage on the skin. This works using the natural SOS mechanism that responds to damage by stopping the cell cycle and activating DNA repair mechanisms. Northwestern is working towards marking this response using a biological circuit hosted by E-coli, they are using GFP as an indicator. We partnered with Northwestern to work towards getting a biobrick (BBa_J04454) they planned on using as part of an internal reference control system to function properly. They were having trouble transforming the biobrick into E-coli using the DH5ɑ strain, so we reattempted the transformation using our own strain of E-coli, genehog. The transformation was successful.

To ensure the success of the part and the functionality of the mCherry gene that was needed by Northwestern, we conducted a fluorescence characterization tracking expression over a period of 4 hours. This yielded positive results. A two-tailed t-test of 0.05 significance between Hour 0 and Hour 3 produced a p-value of 0.000135. This indicates there was significant expression of the mCherry gene over the course of the experiment. To learn more about our collaboration with Northwestern University and the details of their project, click here.

Results












Purdue





The Purdue University iGEM team is working on combating the M. oryzae fungal infection which infects rice plants and results in a significantly decreased crop yield. In order to fight M. oryzae’s ability to bypass the rice plants’ immune response, Purdue is attempting to instigate a preemptive response. To learn more about the project click here..

We conference called with Purdue and discussed troubleshooting our respective projects, they offered us advice on protocol alterations that might help with our characterization of BBa_K584000. In turn, we offered advice on trouble they were having with the formatting of their website.







Jillin China





The Jillin iGEM team set out to develop a system to sense and then subsequently inhibit Candida albicans infection, what is more commonly referred to as a yeast infection. We conferenced with Jillin towards the beginning of both our projects and discussed potential routes of development we could both take.



Address

University of Nebraska-Lincoln iGEM Team
634 Hamilton Hall
Lincoln, NE 68588-0304

Social Media