Difference between revisions of "Team:OUC-China/Parts"

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{{OUC-China}}
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
 
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
 
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This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<ul style="margin:0">
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            <div style="display:inline;height:45px;color: white;padding:0%;text-decoration:none;position:relative;line-height:33px">PROJECT
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            <div class="sub_menu1"  style="background: white;padding:0px;font-size:17px;position:absolute;left:-24px;top:42px;width:135px">
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Description" style="text-decoration:none;padding:0;left:20px">Description</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Design" style="text-decoration:none;padding:0;">Design</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Results" style="text-decoration:none;padding:0;">Results</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Demonstrate" style="text-decoration:none;padding:0;">Demonstrate</a></div>
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<li class="nav-item2" style="width:150px;height:54px;padding:9px;">
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            <div style="display:inline;height:45px;color: white;padding:0%;text-decoration:none;position:relative">LAB WORK
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Experiments" style="text-decoration:none;padding:0;">Experiment</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Safety" style="text-decoration:none;padding:0;">Safety</a></div>
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                      </div>
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            </div>           
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
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            <div style="display:inline;height:45px;color: white;padding:0%;white-space:nowrap;text-decoration:none;position:relative">MODEL
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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            <div class="sub_menu3"  style="position:absolute;background: white;padding:0px;text-decoration:none;font-size:17px;left:-96px;top:42px;width:260px">
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ADD PARTS
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model" style="text-decoration:none;padding:0;">Overview</a></div>
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</a>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model#ode" style="text-decoration:none;padding:0;">ODE</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model#nt" style="text-decoration:none;padding:0;">Thermodynamic model</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model#sta" style="text-decoration:none;padding:0;">Stablizer</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model#asrna" style="text-decoration:none;padding:0;">Antisense RNA</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Model#md" style="text-decoration:none;padding:0;">Molecular Dynamics</a></div>
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            <div style="display:inline;height:45px;color: white;padding:0%;white-space:nowrap;text-decoration:none;position:relative;">PARTS
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Parts" style="text-decoration:none;padding:0;">List</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Basic_Part" style="text-decoration:none;padding:0;">Basic Part</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Composite_Part" style="text-decoration:none;padding:0;">Composite Part</a></div>
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<li class="nav-item5" style="width:40px;height:54px;padding:9px;padding-left:100px">
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            <div style="display:inline;height:45px;color: white;padding:0%;color: white;white-space:nowrap;text-decoration:none;position:relative;" >HP
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            <div class="sub_menu5" id="header_nav4" style="position:absolute;background: white;font-size:17px;left:-75px;top:42px;width:180px">
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Human_Practices" style="text-decoration:none;padding:0;">Human Practices</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Public_Engagement" style="text-decoration:none;padding:0;">Public Engagement</a></div>
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                </div>
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            <div style="display:inline;height:45px;color: white;padding:0%;color: white;text-decoration:none;position:relative" href="#">TEAM
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Team" style="padding:0;text-decoration:none">Members</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Attributions" style="padding:0;text-decoration:none">Attributions</a></div>
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                <div class="nav-sub-item"><a class="kkk" href="https://2019.igem.org/Team:OUC-China/Medal" style="padding:0;text-decoration:none">Medal requirement</a></div>
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li class="nav-item8" style="width:180px;height:54px;padding:9px;">
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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            <a style="display:inline;height:45px;color: white;padding:0%;color: white;text-decoration:none" href="https://2019.igem.org/Team:OUC-China/Collaborations">COLLABORATIONS</a>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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            <a style="display:inline;height:45px;color: white;padding:0%;color: white;text-decoration:none" href="https://2019.igem.org/Team:OUC-China/Software">SOFTWARE</a>
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<a id="toptop" style="position: absolute; top: -100px;">kkkkk</a>
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<div style="position:absolute;z-index:98;width:100%;background:white"><img src="https://static.igem.org/mediawiki/2019/1/1b/T--OUC-China--partstop.png" style="max-width: 100%;max-height:100%"></div>
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<div style="position:fixed;text-align:center;max-width:6%;z-index:100;top:-10px;left:46%;right:53%"><a href="https://2019.igem.org/Team:OUC-China"><img href="https://2019.igem.org/Team:OUC-China" src="https://static.igem.org/mediawiki/2019/9/9a/T--OUC-China--home_logo.png" style="width:108px;"></a></div>
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</br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br></br>
  
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<div class="text" style="font-weight: bold">This year, OUC-China constructed many well-characterized parts based on our standardized design principle. We hope our parts will make it easier and more efficient for synthetic biologists and future iGEM teams to get the predictable results during using riboswitch. Our parts include six different Tuners so that they can be easily incorporated into a user circuit of interest when constructing modular riboswitch. We also include many modular riboswitches by utilizing four kinds of riboswitches. Additionally, many composite parts was constructed to verify our design principle of modular riboswitch.(named “RiboLegoes” )
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</div></br></br></br></br>
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<th style='background-color: #BEB9C7;column-width: 70px;'><center>Part</center></th>
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<th style='background-color: #BEB9C7;column-width: 200px;'><center>Characteristic</center></th>
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<th style='background-color: #BEB9C7;column-width: 200px;'><center>Description</center></th>
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</tr>
  
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<tr>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;line-height: 35px; '><a href="http://parts.igem.org/Part:BBa_K2904000"> K2904000 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Regulatory</td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Tuner A</td>
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</tr>
  
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<tr>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904001"> K2904001 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Tuner B</td>
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</tr>
  
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904002"> K2904002 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Tuner C</td>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904003"> K2904003 </a></td>
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<ul>
+
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
−
<li>Part Name</li>
+
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Tuner D</td>
−
<li>Part type</li>
+
</tr>
−
<li>Creator</li>
+
 
−
<li>Sequence</li>
+
<tr>
−
<li>Short Description (60 characters on what the DNA does)</li>
+
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904004"> K2904004 </a></td>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
−
<li>Design considerations</li>
+
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;line-height: 35px; '>Tuner E</td>
−
</ul>
+
</tr>
  
−
<p>
+
<tr>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904005"> K2904005 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 200px;line-height: 35px; font-size: 15px;'>Tuner S</td>
 +
</tr>
  
 +
</table>
 +
</center>
 
</div>
 
</div>
 +
<div class="text" style="text-align: justify;text-justify:inter-ideograph;padding-bottom: 100px"> <span style="font-weight: bold">Tuners</span> <br/>
 +
Based on the previous attenuator design, we designed six Tuners. Each Tuner confers a distinct level of riboswitch regulation; Tuners are lettered (A-E) in order of their capability of regulating riboswitch response curve, from strongest to weakest. Besides, a special Tuner was also designed to degrade Stabilizer, reducing the metabolic burden of cells. These basic parts should allow future teams to efficiently construct more “RiboLegoes”.
 +
  </div>
  
 +
<div >
 +
<center>
 +
<table style="line-height: 35px">
 +
<tr>
 +
<th style='background-color: #BEB9C7;column-width: 70px;'><center>Part</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Characteristic</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Description</center></th>
 +
</tr>
  
−
<div class="clear extra_space"></div>
+
<tr>
−
<div class="line_divider"></div>
+
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904010"> K2904010 </a></td>
−
<div class="clear extra_space"></div>
+
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing Tuner A</td>
 +
</tr>
  
−
<div class="column full_size">
+
<tr>
−
<h3>Part Table </h3>
+
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904011"> K2904011 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing Tuner B</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904012"> K2904012 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing Tuner C</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904013"> K2904013 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing Tuner D</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904014"> K2904014 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing Tuner E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904015"> K2904015 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Adda riboswitch containing a different source of Stabilizer and Tuner B</td>
 +
</tr>
 +
   
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904020"> K2904020 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Btub riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904021"> K2904021 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Btub riboswitch containing Tuner E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904022"> K2904022 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Btub riboswitch containing Tuner S</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904030"> K2904030 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular Four U riboswitch</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904040"> K2904040 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Regulatory</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Modular cobalamin riboswitch</td>
 +
</tr>
 +
   
 +
</table>
 +
</center>
 +
</div>   
 +
 
 +
<div class="text" style="text-align: justify;text-justify:inter-ideograph;padding-bottom: 250px"> <span style="font-weight: bold">Modular riboswitches </span> <br/>
 +
Our next collection of parts consist of many modular riboswitches. First, we employing Adda riboswitch to construct modular Adda riboswitches by utilizing different Tuners respectively. Then we selected three typical Tuners to design modular Btub riboswitch based on the original Btub riboswitch. An improved cobalamin riboswitch was constructed to verify our design principle. Finally, one thermodynamic switch was also chosen to engineer modular riboswitch.
 +
  </div>
 +
   
 +
<div >
 +
<center>
 +
<table style='line-height: 35px'>
 +
<tr>
 +
<th style='background-color: #BEB9C7;column-width: 70px;'><center>Part</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Characteristic</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Description</center></th>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904050"> K2904050 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904051"> K2904051 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner B</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904052"> K2904052 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner C</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904053"> K2904053 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner D</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904054"> K2904054 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904055"> K2904055 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Adda riboswitch containing a different source Stabilizer</td>
 +
</tr>
 +
   
 +
 
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904022"> K2904022 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904030"> K2904030 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904040"> K2904040 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Device</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner S</td>
 +
</tr>
 +
   
 +
</table>
 +
</center>
 +
</div>   
 +
 
 +
<div class="text" style="text-align: justify;text-justify:inter-ideograph;padding-bottom: 230px"> <span style="font-weight: bold">Reporter gene regulated by modular riboswitch  </span> <br/>
 +
To demonstrate that our modular riboswitch system can work with different proteins, we constructed this collection. Different riboswitches was utilized, including three kinetic switches: Adda riboswitch, Btub riboswitch, cobalamin biosensor, and one thermodynamic switch: Four U riboswitch. What’s more, we used three different kinds of GOI: sfGFP, mRFP1 and EYFP. 
 +
  </div>
 +
 
 +
<div >
 +
<center>
 +
<table style='line-height: 35px'>
 +
<tr>
 +
<th style='background-color: #BEB9C7;column-width: 70px;'><center>Part</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Characteristic</center></th>
 +
<th style='background-color: #BEB9C7;column-width: 200px;'><center>Description</center></th>
 +
</tr>
 +
   
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904020"> K2904020 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing STA81 and Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904021"> K2904021 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing STA129 and Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904092"> K2904092 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Four U riboswitch</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904100"> K2904100 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Measurement</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>Improved vitamin B12 biosensor, composed by modular cobalamin riboswitch in front of an mRFP1</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904120"> K2904120 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904121"> K2904121 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing Tuner B</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904122"> K2904122 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing Tuner C</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904123"> K2904123 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing Tuner D</td>
 +
</tr>
 +
   
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904124"> K2904124 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing Tuner E</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904125"> K2904125 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Adda riboswitch containing a different source of Stabilizer and Tuner B</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904126"> K2904126 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>EYFP regulated by modular Adda riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904130"> K2904130 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Btub riboswitch containing Tuner A</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904131"> K2904131 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Btub riboswitch containing Tuner E</td>
 +
</tr>
 +
   
 +
<tr>
 +
<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904132"> K2904132 </a></td>
 +
<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>Composite</td>
 +
<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>sfGFP regulated by modular Btub riboswitch containing Tuner S</td>
 +
</tr>
 +
 
 +
</table>
 +
</center>
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<div class="text" style="text-align: justify;text-justify:inter-ideograph;padding-bottom: 400px"> <span style="font-weight: bold">aTc inducible sfGFP regulated by modular riboswitch.</span> <br/>
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We further include a collection of aTc-inducible sfGFP reporter constructs regulated by modular riboswitch, all under the control of the Ptet promoter. These parts allowed us to characterize the response curve of modular riboswitch. We successfully demonstrated distinct expression levels of sfGFP by changing different Tuners or riboswitches.
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<th style='background-color: #BEB9C7;column-width: 200px;'><center>Characteristic</center></th>
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<th style='background-color: #BEB9C7;column-width: 200px;'><center>Description</center></th>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904140"> K2904140 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>RNA</td>
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<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>asRNA helping modular Adda riboswitch turn off gene expression</td>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904141"> K2904141 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>RNA</td>
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<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>asRNA helping modular Adda riboswitch turn off gene expression</td>
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</tr>
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<td style='background-color: #EAE8EE;column-width: 70px; font-size: 15px;'><a href="http://parts.igem.org/Part:BBa_K2904142"> K2904142 </a></td>
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<td style='background-color: #E3E0EA;column-width: 200px; font-size: 15px;'>RNA</td>
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<td style='background-color: #E3E0EA;column-width: 400px; font-size: 15px;'>asRNA helping modular Btub riboswitch turn on gene expression</td>
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  <div class="text" style="text-align: justify;text-justify:inter-ideograph;"> <span style="font-weight: bold">asRNA  </span> <br/>To control the riboswitches without medium replacement for removing the inducer, we used the model to design different asRNAs which target different regions to activate or deactivate the riboswitch. These parts can achieve these functionality that expand another method to regulate on-off state of riboswitch besides the ligand.
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<groupparts>iGEM19 OUC-China</groupparts>
 
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Latest revision as of 08:23, 30 November 2019

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This year, OUC-China constructed many well-characterized parts based on our standardized design principle. We hope our parts will make it easier and more efficient for synthetic biologists and future iGEM teams to get the predictable results during using riboswitch. Our parts include six different Tuners so that they can be easily incorporated into a user circuit of interest when constructing modular riboswitch. We also include many modular riboswitches by utilizing four kinds of riboswitches. Additionally, many composite parts was constructed to verify our design principle of modular riboswitch.(named “RiboLegoes” )




Part
Characteristic
Description
K2904000 Regulatory Tuner A
K2904001 Regulatory Tuner B
K2904002 Regulatory Tuner C
K2904003 Regulatory Tuner D
K2904004 Regulatory Tuner E
K2904005 Regulatory Tuner S
Tuners
Based on the previous attenuator design, we designed six Tuners. Each Tuner confers a distinct level of riboswitch regulation; Tuners are lettered (A-E) in order of their capability of regulating riboswitch response curve, from strongest to weakest. Besides, a special Tuner was also designed to degrade Stabilizer, reducing the metabolic burden of cells. These basic parts should allow future teams to efficiently construct more “RiboLegoes”.
Part
Characteristic
Description
K2904010 Regulatory Modular Adda riboswitch containing Tuner A
K2904011 Regulatory Modular Adda riboswitch containing Tuner B
K2904012 Regulatory Modular Adda riboswitch containing Tuner C
K2904013 Regulatory Modular Adda riboswitch containing Tuner D
K2904014 Regulatory Modular Adda riboswitch containing Tuner E
K2904015 Regulatory Modular Adda riboswitch containing a different source of Stabilizer and Tuner B
K2904020 Regulatory Modular Btub riboswitch containing Tuner A
K2904021 Regulatory Modular Btub riboswitch containing Tuner E
K2904022 Regulatory Modular Btub riboswitch containing Tuner S
K2904030 Regulatory Modular Four U riboswitch
K2904040 Regulatory Modular cobalamin riboswitch
Modular riboswitches
Our next collection of parts consist of many modular riboswitches. First, we employing Adda riboswitch to construct modular Adda riboswitches by utilizing different Tuners respectively. Then we selected three typical Tuners to design modular Btub riboswitch based on the original Btub riboswitch. An improved cobalamin riboswitch was constructed to verify our design principle. Finally, one thermodynamic switch was also chosen to engineer modular riboswitch.
Part
Characteristic
Description
K2904050 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner A
K2904051 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner B
K2904052 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner C
K2904053 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner D
K2904054 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing Tuner E
K2904055 Device aTc inducible sfGFP regulated by modular Adda riboswitch containing a different source Stabilizer
K2904022 Device aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner A
K2904030 Device aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner E
K2904040 Device aTc inducible sfGFP regulated by modular Btub riboswitch containing Tuner S
Reporter gene regulated by modular riboswitch
To demonstrate that our modular riboswitch system can work with different proteins, we constructed this collection. Different riboswitches was utilized, including three kinetic switches: Adda riboswitch, Btub riboswitch, cobalamin biosensor, and one thermodynamic switch: Four U riboswitch. What’s more, we used three different kinds of GOI: sfGFP, mRFP1 and EYFP.
Part
Characteristic
Description
K2904020 Composite sfGFP regulated by modular Adda riboswitch containing STA81 and Tuner A
K2904021 Composite sfGFP regulated by modular Adda riboswitch containing STA129 and Tuner A
K2904092 Composite sfGFP regulated by modular Four U riboswitch
K2904100 Measurement Improved vitamin B12 biosensor, composed by modular cobalamin riboswitch in front of an mRFP1
K2904120 Composite sfGFP regulated by modular Adda riboswitch containing Tuner A
K2904121 Composite sfGFP regulated by modular Adda riboswitch containing Tuner B
K2904122 Composite sfGFP regulated by modular Adda riboswitch containing Tuner C
K2904123 Composite sfGFP regulated by modular Adda riboswitch containing Tuner D
K2904124 Composite sfGFP regulated by modular Adda riboswitch containing Tuner E
K2904125 Composite sfGFP regulated by modular Adda riboswitch containing a different source of Stabilizer and Tuner B
K2904126 Composite EYFP regulated by modular Adda riboswitch containing Tuner A
K2904130 Composite sfGFP regulated by modular Btub riboswitch containing Tuner A
K2904131 Composite sfGFP regulated by modular Btub riboswitch containing Tuner E
K2904132 Composite sfGFP regulated by modular Btub riboswitch containing Tuner S
aTc inducible sfGFP regulated by modular riboswitch.
We further include a collection of aTc-inducible sfGFP reporter constructs regulated by modular riboswitch, all under the control of the Ptet promoter. These parts allowed us to characterize the response curve of modular riboswitch. We successfully demonstrated distinct expression levels of sfGFP by changing different Tuners or riboswitches.
Part
Characteristic
Description
K2904140 RNA asRNA helping modular Adda riboswitch turn off gene expression
K2904141 RNA asRNA helping modular Adda riboswitch turn off gene expression
K2904142 RNA asRNA helping modular Btub riboswitch turn on gene expression
asRNA
To control the riboswitches without medium replacement for removing the inducer, we used the model to design different asRNAs which target different regions to activate or deactivate the riboswitch. These parts can achieve these functionality that expand another method to regulate on-off state of riboswitch besides the ligand.