Difference between revisions of "Team:OUC-China/Human Practices"
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/41/T--OUC-China--hp_2-2.jpg | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/41/T--OUC-China--hp_2-2.jpg | ||
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| − | <div class='text'>Figure 1: Why our work is responsible for the world?</div></br> | + | <div class='text' style="text-align:center>Figure 1: Why our work is responsible for the world?</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'>1.1 Ensure the safety of our project</div></br> | <div class='text'>1.1 Ensure the safety of our project</div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/a/a7/T--OUC-China--hpf9.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/a/a7/T--OUC-China--hpf9.png | ||
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| − | <div class='text'>Figure 2: We have strict safety regulations and safe operation.</div></br> | + | <div class='text' style="text-align:center>Figure 2: We have strict safety regulations and safe operation.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'></div></br> | <div class='text'></div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/f/fa/T--OUC-China--tui.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/f/fa/T--OUC-China--tui.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 3: Publish article in WeChat platform.</div></br> | + | <div class='text' style="text-align:center>Figure 3: Publish article in WeChat platform.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'></div></br> | <div class='text'></div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/c/c2/T--OUC-China--lulu.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/c/c2/T--OUC-China--lulu.png | ||
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| − | <div class='text'>Figure 4: We taught them basic microbial experimental operations and on the right is their work.</div></br> | + | <div class='text' style="text-align:center>Figure 4: We taught them basic microbial experimental operations and on the right is their work.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'>2. Explore the values of fundamental research</div></br> | <div class='text'>2. Explore the values of fundamental research</div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/6/61/T--OUC-China--hp2-3.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/6/61/T--OUC-China--hp2-3.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 5: Why our work is good for the world?</div></br> | + | <div class='text' style="text-align:center>Figure 5: Why our work is good for the world?</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'>2.1 Establish links with biotechnology companies</div></br> | <div class='text'>2.1 Establish links with biotechnology companies</div></br> | ||
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<div class='text'>This year, the track which OUC-China selected was Foundational Advance. Given that all basic researches aim at application and benefiting for human beings, the biggest problem we have to face is how to transform our basic research into useful and beneficial application. To verify our project is useful for the world, we communicated with some biological companies who are good at transforming basic researches into practical results.</div></br> | <div class='text'>This year, the track which OUC-China selected was Foundational Advance. Given that all basic researches aim at application and benefiting for human beings, the biggest problem we have to face is how to transform our basic research into useful and beneficial application. To verify our project is useful for the world, we communicated with some biological companies who are good at transforming basic researches into practical results.</div></br> | ||
<div class='text'>We visited some relevant local biotech companies such as Qingdao Bright Moon Seaweed Group Co.LTD and Qingdao youdu biotechnology Co.LTD. We introduced our project to the stakeholders and also gathered many good suggestions. More importantly, they applauded our ideas, held great promise potential application of riboswitch and proposed some application of our project on production. Enlightened by the investigation, we ensured our project is good for the world and optimized our project deeply! </div></br> | <div class='text'>We visited some relevant local biotech companies such as Qingdao Bright Moon Seaweed Group Co.LTD and Qingdao youdu biotechnology Co.LTD. We introduced our project to the stakeholders and also gathered many good suggestions. More importantly, they applauded our ideas, held great promise potential application of riboswitch and proposed some application of our project on production. Enlightened by the investigation, we ensured our project is good for the world and optimized our project deeply! </div></br> | ||
| − | <div class='text'>For more details about optimizing our project, see the Gold Human Practices | + | <div class='text'>For more details about optimizing our project, see the Gold Human Practices Page（Get inspiration and optimize our project）.</div></br> |
<div class='text'>2.1.1 Qingdao Bright Moon Seaweed Group Co. LTD. | <div class='text'>2.1.1 Qingdao Bright Moon Seaweed Group Co. LTD. | ||
Invited by the researchers, we arrived at Qingdao Bright Moon Seaweed Group Co. LTD and visited State Key Laboratory of Bioactive Seaweed Substances. It is a leading enterprise in the Marine biological industry of Shandong province, and the largest seaweed biological products enterprise in the world. Many researchers work in the biological company, developing marine cosmetics, marine food and other high-tech products which are sold all over the country. So it’s necessary to ask them how to explore the value of basic research. During the process of communicating with them, the company's research and development staff praised our project and said that our worry about its usefulness were misplaced. They agreed with the potential applications of riboswitches, which could be greatly expanded if they were developed as modular components. In addition, they encouraged us to improve the regulatory capacity of riboswitch. Finally, to our delight, they mentioned that they would like to apply riboswitch to production. | Invited by the researchers, we arrived at Qingdao Bright Moon Seaweed Group Co. LTD and visited State Key Laboratory of Bioactive Seaweed Substances. It is a leading enterprise in the Marine biological industry of Shandong province, and the largest seaweed biological products enterprise in the world. Many researchers work in the biological company, developing marine cosmetics, marine food and other high-tech products which are sold all over the country. So it’s necessary to ask them how to explore the value of basic research. During the process of communicating with them, the company's research and development staff praised our project and said that our worry about its usefulness were misplaced. They agreed with the potential applications of riboswitches, which could be greatly expanded if they were developed as modular components. In addition, they encouraged us to improve the regulatory capacity of riboswitch. Finally, to our delight, they mentioned that they would like to apply riboswitch to production. | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/8/8b/T--OUC-China--hpf4.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/8/8b/T--OUC-China--hpf4.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 6: We went to the labs of Qingdao Bright Moon Seaweed Group Co.LTD and learned about their current researches.</div></br> | + | <div class='text' style="text-align:center>Figure 6: We went to the labs of Qingdao Bright Moon Seaweed Group Co.LTD and learned about their current researches.</div></br> |
</div></br> | </div></br> | ||
<div class='text'>2.1.2 Qingdao Youdu Biotechnology Co.LTD. | <div class='text'>2.1.2 Qingdao Youdu Biotechnology Co.LTD. | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/d/d2/T--OUC-China--HPf3.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/d/d2/T--OUC-China--HPf3.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 7: Qingdao Youdu Biotechnology Co.LTD applauded our system.</div></br> | + | <div class='text' style="text-align:center>Figure 7: Qingdao Youdu Biotechnology Co.LTD applauded our system.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'>Summary </div></br> | <div class='text'>Summary </div></br> | ||
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<div class='text'>After communicating with biotechnology companies, we also wonder whether other teams and labs focus on the values of basic researches and how they transform it into useful application. So we took part in many conferences. Fortunately, our team was invited to the 5th Synthetic Biology Young Scholar Forum. By communicating with Professor Zhang, we received positive feedback. He praised our project and said that the modular riboswitch approach we proposed can expand the RNA device toolbox, which will be of great use to the biochemical engineering. We were inspired greatly by the fantastic ideas from successful cases and also benefit a lot from the conversations. </div></br> | <div class='text'>After communicating with biotechnology companies, we also wonder whether other teams and labs focus on the values of basic researches and how they transform it into useful application. So we took part in many conferences. Fortunately, our team was invited to the 5th Synthetic Biology Young Scholar Forum. By communicating with Professor Zhang, we received positive feedback. He praised our project and said that the modular riboswitch approach we proposed can expand the RNA device toolbox, which will be of great use to the biochemical engineering. We were inspired greatly by the fantastic ideas from successful cases and also benefit a lot from the conversations. </div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/d/d4/T--OUC-China--HPf4.png" style="max-width:80%"></div></br> | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/d/d4/T--OUC-China--HPf4.png" style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 8: We participated in the 5th Synthetic Biology Young Scholar Forum.</div></br> | + | <div class='text' style="text-align:center>Figure 8: We participated in the 5th Synthetic Biology Young Scholar Forum.</div></br> |
<div class='text'>Furthermore, we had the honor to visit Marine Institute, Chinese Academy of Sciences. Under the guidance of the researchers, we visited the laboratory and learned about the history of the laboratory as well as the results of the innovative research on Marine fish germplasm. The researchers also encouraged us to combine our fundamental research with industry, which was an affirmation of our project. They are willing to apply our projects to their labs in the future.</div></br> | <div class='text'>Furthermore, we had the honor to visit Marine Institute, Chinese Academy of Sciences. Under the guidance of the researchers, we visited the laboratory and learned about the history of the laboratory as well as the results of the innovative research on Marine fish germplasm. The researchers also encouraged us to combine our fundamental research with industry, which was an affirmation of our project. They are willing to apply our projects to their labs in the future.</div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/e/ed/T--OUC-China--HPf5.jpeg | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/parts/e/ed/T--OUC-China--HPf5.jpeg | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 9: We had the honor to visit Marine Institute, Chinese Academy of Sciences.</div></br> | + | <div class='text' style="text-align:center>Figure 9: We had the honor to visit Marine Institute, Chinese Academy of Sciences.</div></br> |
<div class='text'>In order to communicate and cooperate with other iGEM teams better, we participated in the China regional iGEM Exchange Meeting (CCiC), an influential symposium. We publicized our project and related activities better and deeply! At the same time, we understood the forefront of the field of synthetic biology. Besides, some suggestions inspired us to optimize our project.</div></br> | <div class='text'>In order to communicate and cooperate with other iGEM teams better, we participated in the China regional iGEM Exchange Meeting (CCiC), an influential symposium. We publicized our project and related activities better and deeply! At the same time, we understood the forefront of the field of synthetic biology. Besides, some suggestions inspired us to optimize our project.</div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/3/32/T--OUC-China--hpf.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/3/32/T--OUC-China--hpf.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 10: we participated in the China regional iGEM Exchange Meeting (CCiC), an influential symposium.</div></br> | + | <div class='text' style="text-align:center>Figure 10: we participated in the China regional iGEM Exchange Meeting (CCiC), an influential symposium.</div></br> |
<div class='text'>In addition to the conferences above, we also participated in many small seminars organized by iGEMers. Undoubtedly, these are very meaningful and valuable!</div></br> | <div class='text'>In addition to the conferences above, we also participated in many small seminars organized by iGEMers. Undoubtedly, these are very meaningful and valuable!</div></br> | ||
<div class='text'>For more details, see the Collaboration. </div></br> | <div class='text'>For more details, see the Collaboration. </div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/42/T--OUC-China--hpf8.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/42/T--OUC-China--hpf8.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 11: This year, we continued the style of last year and added the new content og comic book.</div></br> | + | <div class='text' style="text-align:center>Figure 11: This year, we continued the style of last year and added the new content og comic book.</div></br> |
<div class='text'>For more details, see the Public Engagement page. </div></br> | <div class='text'>For more details, see the Public Engagement page. </div></br> | ||
<div class='text'></div></br> | <div class='text'></div></br> | ||
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<div class='text'>During reaching out to the communities, we found that many people were interested in microbial and molecular experiments but had no chance to come in the biological lab. iGEM encouraged us to spread synthetic biology to the public by developing new tools. It’s also our responsibility to get more people to experience lab life. With reference to the results of the questionnaire and use VR technology, a great synthetic biology laboratory was built! It’s a golden opportunity for amateurish people to get in touch with laboratory. We have carried on the sharing and the propaganda in the school, has obtained the extremely high praise! We look forward to sharing with you on site this collection of many of our passion and wisdom of VR creation! And expect some feedback. We also recorded a video about VR and it will tell you something about our great idea! We also recorded a video about VR and it will tell you something about our great idea!</div></br> | <div class='text'>During reaching out to the communities, we found that many people were interested in microbial and molecular experiments but had no chance to come in the biological lab. iGEM encouraged us to spread synthetic biology to the public by developing new tools. It’s also our responsibility to get more people to experience lab life. With reference to the results of the questionnaire and use VR technology, a great synthetic biology laboratory was built! It’s a golden opportunity for amateurish people to get in touch with laboratory. We have carried on the sharing and the propaganda in the school, has obtained the extremely high praise! We look forward to sharing with you on site this collection of many of our passion and wisdom of VR creation! And expect some feedback. We also recorded a video about VR and it will tell you something about our great idea! We also recorded a video about VR and it will tell you something about our great idea!</div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/6/61/T--OUC-China--vrhand.png" style="max-width:80%"></div></br> | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/6/61/T--OUC-China--vrhand.png" style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 12:The lab scene in VR.</div></br> | + | <div class='text' style="text-align:center>Figure 12:The lab scene in VR.</div></br> |
<div class='text'>In addition, considering the availability and portability of the equipment, we have also created a mini lab to make it easier to share lab life with the public. We look forward to anyone who is interested in microbiology and molecular experiments to join us! We shared our microlab with the iGEM team and they gave us great advice and feedback!</div></br> | <div class='text'>In addition, considering the availability and portability of the equipment, we have also created a mini lab to make it easier to share lab life with the public. We look forward to anyone who is interested in microbiology and molecular experiments to join us! We shared our microlab with the iGEM team and they gave us great advice and feedback!</div></br> | ||
<div class='text'>For more details, see the Public Engagement page. </div></br> | <div class='text'>For more details, see the Public Engagement page. </div></br> | ||
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<div class='text'>For more details and get the PDF, see the Experimental manual. </div></br> | <div class='text'>For more details and get the PDF, see the Experimental manual. </div></br> | ||
<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/4b/T--OUC-China--igemexperimentbook.png" style="max-width:60%"></div></br> | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/4/4b/T--OUC-China--igemexperimentbook.png" style="max-width:60%"></div></br> | ||
| − | <div class='text'>Figure 13:The cover of the lab manual.</div></br> | + | <div class='text' style="text-align:center>Figure 13:The cover of the lab manual.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'>1.6 WeChat Platform</div></br> | <div class='text'>1.6 WeChat Platform</div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/5/53/T--OUC-China--HP4.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/5/53/T--OUC-China--HP4.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 14: We make good use of our WeChat platform to spread the values of iGEM.</div></br> | + | <div class='text' style="text-align:center>Figure 14: We make good use of our WeChat platform to spread the values of iGEM.</div></br> |
<div class='text'></div></br> | <div class='text'></div></br> | ||
<div class='text'></div></br> | <div class='text'></div></br> | ||
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<div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/5/52/T--OUC-China--hpf11.png | <div class='text'style="text-align:center"><img src="https://static.igem.org/mediawiki/2019/5/52/T--OUC-China--hpf11.png | ||
" style="max-width:80%"></div></br> | " style="max-width:80%"></div></br> | ||
| − | <div class='text'>Figure 15: Inspired by Professor Li Yun, we utilized asRNA to regulate the on-off state of riboswitch.</div></br> | + | <div class='text' style="text-align:center>Figure 15: Inspired by Professor Li Yun, we utilized asRNA to regulate the on-off state of riboswitch.</div></br> |
<div class='text'>For more details, see the Results page. </div></br> | <div class='text'>For more details, see the Results page. </div></br> | ||
<div class='text'></div></br> | <div class='text'></div></br> | ||
Revision as of 02:38, 22 October 2019
Bronze
Silver
Gold
Bronze
1. Project inspiration and description
Riboswitch is RNA-based regulatory tool that specifically modulates the expression of a gene or operon. It holds great potential as regulatory element in various applications (e.g. bioenergetics, biofuels, therapy, bioremediation and biochemical engineering). However, by referencing the project of Paris_Bettencourt in 2005 and other study about riboswitch, we found that it can’t be regarded as a modular device. The three problems below make the riboswitch quite hard to design and employ to the application.
○First, due to the context-dependent performance of riboswitch, its structure is easily damaged by the GOI, leading to the failure of responsive to ligand, so it can’t be a plug and play device.
○Second, existing riboswitch only has simple response curve. It’s restricted by the limited dynamic range and cannot achieve multi-level regulation. So riboswitch function is expected to enhance.
○Third, because the ligand of riboswitch is often hard to degrade in the experimental culture system, it often makes the system away from your prediction. So it’s not easily toggle between the on and off state.
Inspired by past literature and iGEM projects, we know that it's time to make modular riboswitch. This year, we proposed a principle to make modular riboswitch-”RiboLego”. Through our approach, riboswitch can be considered as a modular device whose function is enhanced and varied. So this project is a useful application of synthetic biology.
For more details, see the Project Inspiration and Description page.
Silver
1. Attach importance to bio-safety
1. Create many tools derived from the project
1.1 RiboFold
This year, OUC-China proposed a approach to design modular riboswitch rationally. However, when we tried to explain our projects to more people, we found that it’s difficult to fully understand, especially for some amateurish people. In order to let more people understand the principles of biology in an interesting and effective way, we are creating a game - meticulously. The game aims to allow users to build more riboswitches by adding sequence of expression platform while keeping the structure of the riboswitch intact. It will offer you the aptamer region of riboswitch and four buttons which respectively represent four bases. By continuously clicking the buttons, users can add the bases and complemented the expression platform of riboswitch. At the same time, the changed structure of riboswitch will appear on the screen. We believe that our game will offers the users an opportunity to randomly change the structure of riboswitch and construct more riboswitches. They can understand that the structure of riboswitch is so fragile that can easily influenced by the its sequence. And by playing our game, more people can understand our project very well! We will continue to improve it in order to achieve the best presentation effect!
1.2 Comic book
Ocean University of China published a comic book on synthetic biology in 2017. In 2018, the E. coli SPACESHIP comic book series began to cooperate on the iGEM official website and achieved high praise. This year, OUC-China continued the style of last year and added the new content. We have draw three more chapters, respectively including Riboswitch, Quorum Sensing and Safety.More foreign teams are willing to help us diversify the language of the comic book. At the same time, we cooperate with many domestic and foreign teams, and we are very honored that they are willing to use our comic book to promote the science of synthetic biology. It is worth mentioning that these teams also give us some good feedback about the effects of comic book E.coli SPACESHIP. we hope everyone to actively participate in science popularization, achieving the purpose of improving the depth and breadth of science popularization, strengthening the inheritance of synthetic biology.
For more details, see the Public Engagement page.
1.4 VR&Mini-lab: the real life of lab
During reaching out to the communities, we found that many people were interested in microbial and molecular experiments but had no chance to come in the biological lab. iGEM encouraged us to spread synthetic biology to the public by developing new tools. It’s also our responsibility to get more people to experience lab life. With reference to the results of the questionnaire and use VR technology, a great synthetic biology laboratory was built! It’s a golden opportunity for amateurish people to get in touch with laboratory. We have carried on the sharing and the propaganda in the school, has obtained the extremely high praise! We look forward to sharing with you on site this collection of many of our passion and wisdom of VR creation! And expect some feedback. We also recorded a video about VR and it will tell you something about our great idea! We also recorded a video about VR and it will tell you something about our great idea!
For more details, see the Software page.
1.5 Experiment manual
Invited by our teachers in Experiments Center, we edited an experimental manual including some basic microbiological operations and molecular operations independently. Our teacher wants to set up an experiment course with this experiment manual. We hope that our experimental operations will provide a reference to other students. We are very willing to share to realize the inheritance of iGEM.
For more details and get the PDF, see the Experimental manual.
